紫花苜蓿不同品种对拟枝孢镰刀菌的抗性评价  

Resistance evaluation of different alfalfa varieties to Fusarium sporotrichioides

作　　者：潘龙其 张丽[1] 田进山 袁庆华[1] 王瑜[1] 苗丽宏[1] 

机构地区：[1]中国农业科学院北京畜牧兽医研究所,北京100193 [2]宜昌市农业科学研究院,湖北宜昌443004

出　　处：《草业学报》2016年第5期95-101,共7页Acta Prataculturae Sinica

基　　金：公益性行业(农业)科研专项项目(201303057)资助

摘　　要：在温室盆栽30个紫花苜蓿品种45d后,将拟枝孢镰刀菌接种于幼苗根部土壤中,10d根据根部病情指数评定抗病性,筛选抗性后选择抗病和感病品种再次接种,于4,8和12h时测定与抗性相关酶的活性。结果表明,不同品种间抗性差异显著,其中耐病品种为晋南苜蓿、驯鹿、PG塞特、苏联二号4个,占测定品种总数的13.3%;高感品种为新疆大叶、中苜一号、陇中苜蓿、北疆苜蓿、皇冠、甘肃苜蓿和萨兰斯7个,占23.3%;其余的均为感病品种,占63.4%;未发现抗病和免疫品种。酶活性变化测定表明,对于接菌与未接菌的供试耐病和高感苜蓿品种,不同品种间CAT、SOD、PAL的活性存在一定差异;总体接菌植株的酶活性高于对照,且耐病品种的酶活性高于感病品种,酶活性在接菌后4~8d呈上升趋势,8~12d呈下降趋势;接菌植株CAT活性较对照增幅明显,SOD、PAL活性较其对照增幅不明显。The present study evaluated the resistance of 30 alfalfa varieties to Fusarium sporotrichioides by in oculation of 45 d old seedlings in a greenhouse. Resistant and susceptible varieties were selected based on the root disease index after 10 days of inoculation and were further evaluated by inoculation pathogen and measur ing enzymes activities after 4,8 and 12 hours inoculation. The results showed that there were four resistant va rieties： Jinnan, Xunlu, PG Saite and Sulian No. 2, which together accounted for 13. 3%. There were seven highly sensitive varieties： Xinjiangdaye, Zhongmu No. 1, Longzhong, Beijiang, Huangguan, Gansu and Salan si, which together accounted for 23.3%. The remaining varieties were all sensitive and together accounted for 63.4%. There were no resistant or immune varieties. Measurement of enzyme activities showed highly signifi cant variations in CAT, SOD and PAL activities among the different varieties, for both the inoculated samples and for the controls without inoculation. Enzyme activities in inoculated plants were higher than those of con trol plants and enzyme activities in the resistant varieties were higher than those of susceptible varieties. En zyme activity increased after 4-8 d inoculation and decreased after 8-12 d inoculation. CAT activity of the in oculated plants significantly increased compared to the control, while SOD and PAL activities did not signifi cantly increase.

关 键 词：拟枝孢镰刀菌 抗性评价 酶活测定 

分 类 号：S435.4[农业科学—农业昆虫与害虫防治]