TGF-β1对大鼠肩袖损伤修复术后腱-骨愈合的影响  

Effects of TGF-β_1 on early tendon-bone healing after reconstruction of rotator cuff tears in rats

作　　者：张冲[1] 李莉[2] 

机构地区：[1]河北省中医院骨科,石家庄050000 [2]河北医科大学基础医学院,组织学与胚胎学教研室,石家庄050017

出　　处：《医用生物力学》2016年第2期167-170,187,共5页Journal of Medical Biomechanics

基　　金：河北省医学科学研究重点课题(ZL20140293)

摘　　要：目的评价转化生长因子β_1(transforming growth factorβ_1,TGF-β_1)对Wistar大鼠肩袖损伤重修复术后早期腱-骨界面愈合的影响。方法 54只Wistar大鼠,取双侧大结节止点处横断撕裂肩袖冈上肌腱全层造模,6周后重建肩袖止点,腱骨界面注射以纤维蛋白胶为载体的TGF-β_1缓释体诱导成骨。随机分为高、低剂量组和单纯手术对照组。术后1、2、3周随机分组处死动物,取肩袖标本作生物力学检测。结果肉眼观察各组断裂点均在肌腱缝合处以内。高剂量组最大抗拉强度、最大载荷百分比、刚度及最大横断面面积与低剂量组和对照组比较有显著差异(P<0.05)。低剂量组最大载荷百分比、最大抗拉强度、刚度、刚度百分比、最大横断面面积与对照组比较有显著差异(P<0.05)。高剂量TGF-β_1在肩袖损伤修复早期效果较佳,腱-骨界面的抗拉强度及刚度随着康复时间的延长而不断增强。结论 TGF-β_1加强腱-骨界面的最大抗拉强度、最大载荷百分比、刚度及最大横断面面积,诱导断面再生成直止点的复杂结构,促进肩袖损伤的愈合。Objective To evaluate the effect of transforming growth factor β1（ TGF-β1） on early tendon-bone healing after reconstruction of rotator cuff tears in Wistar rats. Methods The models of rotator cuff supraspinatus tendon by full-thickness transverse tears at the greater tuberosity point in bilateral upper limbs of 54 Wistar rats were established and reconstructed. After 6 weeks,TGF-β1induced by fibrin glue was injected at the tendon-bone interface to release osteogenesis. The rats were randomly divided into low-dose TGF-β1group,high-dose TGF-β1group and control group,and then executed randomly at 1st,2nd and 3rd week to make the supraspinatus specimens for biomechanical testing. Results The rupture point was formed within the suture of rotator cuff in each group by visual observation. The maximum tensile strength,maximum loading percentage,stiffness and maximum cross-section area of tendon-bone interface in high-dose group were higher than those in low-dose group and control group（ P〈0. 05）. The maximum loading percentage,maximum tensile strength,stiffness,stiffness percentage and the maximum cross-section area of tendon-bone interface in low-dose group were significantly higher than those in control group（ P〈0. 05）. High-dose TGF-β1could promote the healing during early reconstruction of rotator cuff tears,the tensile strength and stiffness at the tendon-bone interface would increase with the rehabilitation period prolonging. Conclusions Using TGF-β1can increase the maximum tensile strength,maximum loading percentage,stiffness and maximum cross-section area of tendon-bone interface,so as to induce the fractured section to generate complex structure directly,thus promote the healing of rotator cuff tears.

关 键 词：转化生长因子Β1 肩袖损伤 腱-骨愈合 生物力学 

分 类 号：R684.7[医药卫生—骨科学] R318.01[医药卫生—外科学]