辅助性T细胞17／调节性T细胞平衡相关因子在类风湿关节炎中的表达及其与血清中期因子的相关性  

The expression of helper T cells 17/regulatory T cells balance associated factors in rheumatoid arthritis patients and their correlation with serum midkine

作　　者：胡文霞[1] 杨晋[2] 杨新玲[2] 赵绍林[2] 吴惠毅[1] 

机构地区：[1]江苏省连云港市第一人民医院检验科,222002 [2]江苏省连云港市第一人民医院中心实验室,222002

出　　处：《中华风湿病学杂志》2016年第4期224-228,共5页Chinese Journal of Rheumatology

摘　　要：目的探讨RA患者血清中期因子（MK）与辅助性T细胞（Th17）／调节性T细胞免疫平衡相关因子的表达及其相关性。方法应用ELISA法检测60例RA患者（活动期32例和非活动期28例）和30名健康体检者血清中MK水平，流式细胞术（FCM）检测RA患者和健康对照者外周血中IL-17＋CD4＋T细胞（Thl7细胞）与CD25＋CD4＋Foxp3＋T细胞（调节性T细胞）的百分比，RT-PCR检测调节性T细胞和Th17细胞分化的特异性转录因子叉状头转录因子（Foxp3）和维甲酸相关孤核受体转录因子（RORγt）mRNA以及信号转导与转录激活因子（STAT）3和STAT5mRNA的表达水平，多组间采用单因素方差分析，随后采用q检验两两比较，2组间采用t检验，相关性分析采用Pearson相关分析。结果活动RA组、非活动RA和健康对照组外周血调节性T细胞百分比差异有统计学意义（F=129．6，P〈0．01），活动RA组[（1．4±1．0）％]明显低于非活动RA组[（3．6±1．6）％；q=7．92，P〈0．05]和健康对照组[（7．7＋1．7）％；q=22．45，P〈0．05]，差异有统计学意义，非活动RA组也低于健康对照组（q=14．53，P〈0．05），差异有统计学意义；3组Th17细胞百分比差异有统计学意义（F=36．3，P〈0．01），活动RA组Th17（1．84±1．01）％明显高于非活动RA组[（0．71±0．28）％；q=9．59，P〈0．05]和健康对照组[（0．53±0．16）％；q=11．10，P〈0．05]，差异有统计学意义，非活动RA组和健康对照组比较差异无统计学意义（q=1．51，P〉0．05）；RA组RORγt、STAT3mRNA的表达水平明显高于健康对照组，差异有统计学意义（t=5．84，P〈0．01；t=4．52，P〈0．01）；RA组Foxp3和STAT5mRNA水平低于健康对照组，差异有统计学意义（t=6．01，P〈0．01；t=2．18，P〈0．05）。血清MK水平与外周血PBMCsSTAT5mRNA表达呈负相关（r=-0．55，P〈0．01），与Foxp3、RORγt、STAT3mRNA、Th17和调节Objective To investigate the expression of helper T cells （Th）17/regulatory T cells （Treg） balance associated factors in rheumatoid arthritis （RA） patients and their correlation with serum midkine （MK）. Methods A total of 60 RA patients were divided into active RA patients （n=32） and inactive RA patients group （n=28）. MK level in sera was detected by enzyme linked immunosorbent assay （ELISA） in 60 patients with RA and 30 healthy controls （HCs）. The fraction of CD4＋CD25＋FOXP3＋ Treg cells and IL-17~CD4~ Thl7 cells in RA patients and healthy controls were determined by flow cytometry （FCM）, and the expression of Foxp3, RORγt, Signal transducer and activator of transcription （STAT） 3 and STAT5 mRNA were detected with real-time polymerase chain reaction （PCR）. Results were evaluated using ANOVA followed by q tests for comparisons of Thl7 population between active RA patients, inactive RA patients and HCs, t test was used for comparing of Foxp3, RORγt, STAT3, STATS mRNA between RA group and HCs. The correlations between serum MK concentration and peripheral Treg cells, Thl7 cells, Foxp3, RORγt, STAT3, STATS mRNA were analyzed by Pearson＇s correlation analysis. Results The percentages of Treg cells from active RA patients, inactive RA patients and HCs were significantly different （F=129.6, P〈0.01）, the percentages of Treg cells of active RA patients [（1.41 ±1.05）%] were lower than that of the inactive RA patients [（3.6±1.6）%, q =7.92, P〈0.05] and healthy group [（7.7±1.7）%; q=22.45, P〈0.05], and there was significant difference between healthy group and inactive RA group （q=14.53, P〈0.05）. The percentages of Thl7 cells of the three groups were also significantly different （F=36.3, P〈0.01）,the percentage of Thl7 cells of active RA patients [（1.84±1.01）%] was significantly higher than that of inactive RA patients [（0.71±0.28）%; q=9.59, P〈0.05] and healthy group （0.53±0.16）% [（P〈0.05; q=11.10, P〈0.05], there wa

关 键 词：关节炎 类风湿 T淋巴细胞 血清中期因子 信号转导与转录激活因子 

分 类 号：R593.22[医药卫生—内科学]