BHK_(21)细胞对蚀斑法检测乙型脑炎减毒活疫苗结果的影响分析  

Analysis of effect of BHK_(21) cell on result for plaque assay in detection of Japanese encephalitis vaccine,live

作　　者：吕文利[1] 张雪[1] 袁瑾[1] 赵新华[1] 肖健[1] 

机构地区：[1]武汉生物制品研究所有限责任公司乙脑疫苗室,湖北武汉430207

出　　处：《微生物学免疫学进展》2016年第1期18-21,共4页Progress In Microbiology and Immunology

摘　　要：目的分析BHK_(21)细胞对蚀斑法检测乙型脑炎减毒活疫苗系统的影响,降低检测系统误差。方法比较BHK_(21)细胞以不同频次传代培养时的细胞生长状态,以及以不同接种浓度进行疫苗病毒滴度检测时,对检测系统稳定性的影响。结果 BHK_(21)细胞培养4 d传代,其形态良好、边缘光滑、胞质透光性好、细胞分散均匀、细胞活率达到95%以上;BHK_(21)以105细胞/m L浓度接种时,乙型脑炎减毒活疫苗及其病毒滴度参考品的变异系数最小,分别为0.66%和0.64%。结论 BHK_(21)细胞培养4 d传代、以105个/m L浓度接种时用蚀斑法检测病毒滴度系统最稳定,可供乙型脑炎减毒活疫苗滴度检测参考。Objective To analyse the effect of BHK21 cell on result for plaque assay in detection of Japanese encephalitis vaccine,live,and reduce the error in the detection system. Methods To test the effect of the cell growth status of BHK21 cell on the stability of the detection system by comparing different passage frequency under different concentrations of BHK21 cell. Results The better growth cells were obtained with good shape,smooth edge,transparent cytoplasm,uniform distribution,and more than 95% viability,when the BHK21 cell was passaged by four days. The coefficients of variation were minimal for Japanese encephalitis vaccine,live and related virus titer reference,which were 0. 66% and 0. 64%,respectivelly,when BHK21 cells were planted by the concentration of 105/ m L. Conclusion The detection system is the most stable when the BHK21 cell was passaged by four days with the concentration of 105/ m L,which providesa suitable reference for plaque assay in detection of Japanese encephalitis vaccine,live.

关 键 词：BHK21细胞 蚀斑法 传代频次 接种浓度 参考 

分 类 号：R392[医药卫生—免疫学]