高效液相色谱法同时测定复方黄连素片中芍药苷和盐酸小檗碱含量  

Content Determination of Paeoniflorin and Berberine Hydrochloride in Compound Huangliansu Tablets by HPLC

作　　者：李静 钱江[2] 

机构地区：[1]江苏省扬州市食品药品检验检测中心,江苏扬州225009 [2]扬州大学医学院,江苏扬州225001

出　　处：《中国药业》2016年第7期61-63,共3页China Pharmaceuticals

摘　　要：目的建立同时测定复方黄连素片中芍药苷和盐酸小檗碱含量的高效液相色谱法。方法色谱柱为Phenomenex Luna C_（18）柱（250 mm×4.6 mm,5μm）,流动相为0.4%磷酸溶液-乙腈（梯度洗脱）,流速为1.0 m L/min,检测波长为230 nm,柱温为35℃,进样量为10μL。结果芍药苷和盐酸小檗碱的进样质量浓度分别在1.496 1~187.016 0μg/m L（r=0.999 9）和1.788 1~223.512 6μg/m L（r=0.999 9）范围内与峰面积呈良好的线性关系,平均回收率分别为100.58%（RSD=1.29%,n=6）和101.58%（RSD=1.76%,n=6）。结论该法简便准确,可用于同时测定复方黄连素片中芍药苷和盐酸小檗碱的含量。Objective To establish an HPLC method for the content determination of paeoniflorin and berberine hydrochloride in Compound Huangliansu Tablets. Methods The chromatographic column was Phenomenex Luna C_（18） column（ 250 mm × 4. 6 mm, 5 μm）; the mobile phase was composed of 0. 4% phosphoric acid- acetonitrile; the flow rate was 1. 0 m L / min; the detection wavelength was 230 nm;the column temperature was 35 ℃ and the injection volume was 10 μL. Results The linear ranges of paeoniflorin and berberine hydrochloride were 1. 496 1- 187. 016 0 μg / m L（ r = 0. 999 9） and 1. 788 1- 223. 512 6 μg / m L（ r = 0. 999 9） respectively, and their average recovery rates were 100. 58%（ RSD = 1. 29%, n = 6） and 101. 58%（ RSD was 1. 76%, n = 6） respectively. Conclusion The method is simple,accurate and suitable for determining the contents of paeoniflorin and berberine hydrochloride in Compound Huangliansu Tablets.

关 键 词：高效液相色谱法 复方黄连素片 芍药苷 盐酸小檗碱 

分 类 号：R284.1[医药卫生—中药学] R286.0[医药卫生—中医学]