荆防散乙酸乙酯部位对过敏性动物模型和IFN-γ、IL-4水平的影响  

Study on the Mechanism of Antiallergic effect of ethyl acetate extraction of Jingfang Powder in allergic animal models

作　　者：阙昌田 王哲[1] 温桃群 王凤[1] 熊茜[1] 曾知[1] 曾南[1] 

机构地区：[1]成都中医药大学药学院,中药材标准化教育部重点实验室,四川省中药资源系统研究与开发利用重点实验室-省部共建国家重点实验室培育基地,成都611137

出　　处：《中药药理与临床》2016年第1期127-130,共4页Pharmacology and Clinics of Chinese Materia Medica

基　　金：国家基础科学人才培养基金项目(J1310034-09);四川省科技厅青年科技创新研究团队项目(2014TD0007)

摘　　要：目的:研究荆防散乙酸乙酯部位对大鼠、豚鼠过敏性模型及IFN-γ、IL-4水平的影响,初步探讨其抗过敏作用机制与纠正Th1/Th2免疫失衡的关系。方法:采用卵白蛋白合并百白破疫苗致大鼠全身主动过敏反应模型和卵白蛋白致豚鼠哮喘模型,测定大鼠脏器(胸腺、脾)指数及血清IL-4、IFN-γ水平;测定豚鼠全血白细胞计数、血清Ig E水平,及肺泡灌洗液(BALF)中IL-4、IFN-γ水平和总蛋白含量。结果:与模型对照组比较,荆防散乙酸乙酯部位5.0 g生药/kg、2.5 g生药/kg显著降低全身主动过敏反应模型大鼠血清IL-4水平、升高大鼠胸腺指数及血清IFN-γ水平,并能显著降低哮喘模型豚鼠全血白细胞计数和血清Ig E水平;荆防散乙酸乙酯部位2.5 g生药/kg显著对抗哮喘模型豚鼠肺泡灌洗液中IL-4、总蛋白含量的升高及IFN-γ水平的降低。结论:荆防散乙酸乙酯部位抗过敏作用良好,作用机制与上调IFN-γ水平、下调IL-4水平有关,提示其能通过纠正Th1/Th2免疫失衡来抑制过敏反应。Objective： To study the effects of ethyl acetate extraction of Jingfang Powder on IFN-γ,IL-4 and an-tiallergic effect in two kinds of allergic animal models,and reveal the relationship of its mechanism and correct the Th1 / Th2 immune imbalance. Methods： By using the model of active systemic anaphylaxis induced by ovalbumin and DPT vaccine in rats,and the model of asthma induced by ovalbumin in guinea pigs,the index of thymus and splee and the levels of IL-4,IFN-γ in serum in model rats were measured,and the WBC counts in blood,the level of Ig E in serum,and the levels of IL-4,IFN-γ,total albumen in bronchoalveolar lavage fluid（ BALF）,and eosinophils infiltration of lung pathological sample were measured in guinea pigs. Results： Compared with the model control group,the ethyl acetate extraction of Jingfang Powder significantly reduced the level of IL-4 in serum,and increased the index of thymus and the level of IFN-γ in serum at the dose of 5.0,2. 5 g / kg in model rat. Meanwhile,the same dosages of ethyl acetate extraction of Jingfang Powder also significantly reduced WBC counts in blood,,Ig E level in serum,and the numbers of eosinophils in lung tissue in guinea pig. In addition,at the dose of 2. 5 g / kg,it significantly increased the IL-4 level,and decreased total albumen content and the IFN-γ level in BALF in guinea pig. Conclusion： The ethyl acetate extraction of Jingfang powder had good anti-allergic effect,the mechanism of anti-allergic effect may be relate to raise the level of IFN-γand reduce the level of IL-4,namely that in the aspect of correcting allergic reaction of Th1 / Th2 immune imbalances played a certain role.

关 键 词：荆防散 乙酸乙酯部位 抗过敏 Th1/Th2免疫失衡 免疫球蛋白E 

分 类 号：R285.5[医药卫生—中药学] R-332[医药卫生—中医学]