解郁育胞方对慢性应激致大鼠子宫内膜低容受性的机制研究  

Effect of Jieyuyubao Prescription on endometrial receptivity of the chronic stress rats

作　　者：耿会转[1] 周忠明[1] 邓亚丽[1] 周顺长[2] 贺漪[3] 沈健[4] 宋俐[1] 桂玉然[1] 冯利[1] 余皖娟 黎烈荣[1] 张平[1] 

机构地区：[1]湖北中医药大学附属医院,武汉430061 [2]华中科技大学实验动物中心,武汉430030 [3]武汉市第一医院妇产科,武汉430022 [4]武汉市中心医院妇产科,武汉430014

出　　处：《中药药理与临床》2016年第1期152-156,共5页Pharmacology and Clinics of Chinese Materia Medica

基　　金：中医校字([2013]148号)资金资助;"IVF-ET失败女性抑郁症调查及中药疏肝解郁干预的临床研究"

摘　　要：目的:研究解郁育胞方对慢性应激大鼠妊娠率、平均着床点数的影响,以及对血清β-内非肽(β-EP)、子宫内膜血管内皮生长因子(VEGF)及胞饮突的影响,探讨其对慢性应激大鼠子宫内膜容受性的调节机制。方法:80只SPF级SD大鼠按体重分层法随机分为5组:正常对照组、应激组、应激+5.6g/kg解郁育胞方组、应激+11.2g/kg解郁育胞方组、应激+22.4g/kg解郁育胞方组。除正常对照组外,其余4组大鼠建立慢性应激模型,于刺激第15日开始,应激+11.2g/kg解郁育胞方组每日9:00给予解郁育胞方溶液(相当于生药1g/ml)灌胃,给药容量为11.2g/kg;正常对照组、应激组灌服等体积纯化水(11.2g/kg);应激+5.6g/kg解郁育胞方组、应激+22.4g/kg解郁育胞方组分别按应激+11.2g/kg解郁育胞方组折算临床有效剂量的1/2和2倍给药,给药容量5.6g/kg和22.4g/kg,每日1次。5组大鼠分别于第28日16:00按照雌:雄=2:1合笼,经阴道涂片确认妊娠后第4日停药,当日16:00处死大鼠,检测血清β-EP、子宫内膜VEGF含量及子宫内膜胞饮突成熟度。结果:平均妊娠率和着床点数:各组经过10日合笼,应激组和应激+5.6g/kg解郁育胞方组各有1只大鼠仍未妊娠;与应激组相比,应激+11.2g/kg解郁育胞方组和应激+22.4g/kg解郁育胞方组的平均着床点数明显增高。2血清β-EP含量:与正常对照组相比,应激组、应激+5.6g/kg解郁育胞方组的β-EP含量明显升高;应激+11.2g/kg解郁育胞方组、应激+22.4g/kg解郁育胞方组与应激组比较有显著差异;应激+11.2g/kg解郁育胞方组组、应激+22.4g/kg解郁育胞方组的β-EP含量明显低于应激+5.6g/kg解郁育胞方组。VEGF的表达:VEGF主要位于子宫内膜腺上皮细胞的胞浆和部分基质血管内皮细胞的胞浆,部分间质细胞有弱着色,各试验组表达强弱不一。与A正常对照组相比,应激组VEGF的表达明显降低;应激+22.4g/kg解郁育胞方组与应激组相比有显著统计学意义Objective： To study the impact of Jieyuyubao prescription in rats with chronic stress for serum β-EP,endometrial VEGF and pinopodes expression,revealing the chronic stress rats on endometrial receptivity regulatory mechanisms. Methods： 80 SPF SD rats were stratified randomly into 5 groups： normal control group,stress group and stress ＋ 5. 6g / kg Jieyuyubao prescription solution group,stress ＋ 11. 2g / kg Jieyuyubao prescription solution group,stress ＋ 22. 4g / kg Jieyuyubao prescription solution group. In addition to the normal control group,the remaining four groups of rats were established drug chronic stress model. stress ＋ 11. 2g / kg Jieyuyubao prescription solution group daily 9： 00 give Jieyuyubao prescription solution（ equivalent to crude drug 1g / ml） by gavage,dosing capacity for 11. 2g / kg; a normal control group,stress group filling service such as volume purified water（ 11. 2g / kg）; stress ＋ 5. 6g / kg Jieyuyubao prescription solution group and stress ＋22. 4g / kg Jieyuyubao prescription solution group were in stress ＋ 11. 2g / kg Jieyuyubao prescription solution group conversion clinically effective doses of 1 /2 and 2 times to medicine and drug delivery capacity 5. 6g / kg and 22. 4g / kg,daily 1 times. Five groups of rats respectively in the 28 th 16： 00 in accordance with the female： male = 2： 1） caged and the vaginal smear pregnancy is confirmed after the 4th withdrawal.At 16： 00 the same day killed rats,serum β-EP,uterine endometrial VEGF levels and endometrial pinocytic process maturity. Results： The average pregnancy rate and the number of implantation： 10 rats in each group were not pregnant at each group of 1 rats. The average number of implantation points in group stress ＋ 22. 4g / kg Jieyuyubao prescription solution group and stress ＋ 11. 2g / kg Jieyuyubao prescription solution group was significantly higher than that in stress ＋ 5. 6g / kg Jieyuyubao prescription solution group（ P 0. 01）. the serum levels of beta endorphine： A

关 键 词：解郁育胞方 慢性应激 子宫内膜 容受性 

分 类 号：R285.5[医药卫生—中药学]