SND1转基因小鼠的构建  

Construction of SND1 Transgenic Mice

作　　者：左志宇[1] 辛灵彪[1] 杨洁[1,2] 王鑫廷[1,2] 

机构地区：[1]天津医科大学基础医学院免疫学系,天津300070 [2]天津医科大学基础医学院医学分子生物学系,天津300070

出　　处：《中国生物工程杂志》2016年第4期97-103,共7页China Biotechnology

基　　金：国家自然科学基金(31125012;31300709)资助项目

摘　　要：目的:构建SND1过表达的转基因小鼠模型。方法:利用对小鼠SND1基因转录本构建SND1过表达载体p Insulator-CAG-3×FLAG-SND1,利用受精卵原核注射技术,将外源线性p Insulator-CAG-3×FLAG-SND1转基因载体注射到受精卵细胞核内,将存活受精卵进行胚胎移植制备SND1转基因小鼠,用PCR、RT-PCR技术鉴定转基因小鼠是否构建成功。结果:成功构建过表达SND1基因的转基因小鼠模型,为进一步研究SND1基因在动物体内的生物学功能奠定基础。Objective：The aim is to establish an animal model of SND1 over-expression transgenic mice.Methods：The mice SND1 gene transcripts were used for form p Insulator-CAG-3 × FLAG-SND1 recombinant vector.Then injected the recombinant vector into fertilized egg by microinjection technology to acquire the transgenic mice.These offspring were identified by PCR.Results：SND1 transgenic mice was constructed successfully and expressed effectively on account of target gene can be transferred by the transgenic mice which was proved by PCR and RT-PCR.These mice could provide an important model for studying the biological function of SND1 gene in vivo.

关 键 词：SND1 载体构建 转基因小鼠 

分 类 号：Q789[生物学—分子生物学]