UVB照射对系统性红斑狼疮自噬相关基因及蛋白表达水平的影响  

The Influence of UVB Irradiation on the Expression Levels of Autophagy Related Proteins and Genes in Systemic Lupus Erythematosus

作　　者：韩露露[1] 钱革[2] 胡桂兰[1] 邓丹琪[1] 

机构地区：[1]昆明医科大学第二附属医院皮肤性病科,云南昆明650101 [2]郑州市儿童医院,河南郑州450000

出　　处：《中国皮肤性病学杂志》2016年第5期445-448,共4页The Chinese Journal of Dermatovenereology

基　　金：国家自然科学基金(81460472);云南省科技厅-昆明医学院联合专项基金资助项目(2014FB050)

摘　　要：目的观察UVB照射对SLE患者的外周血单个核细胞(PBMC)的自噬现象,探讨UVB、自噬对SLE产生的影响。方法应用RT-q PCR,Western blotting技术在基因和蛋白水平测定活动性SLE患者外周血淋巴细胞自噬标志物Uvrag,Beclin1和LC3Ⅱ的表达水平,并通过统计学分析其内在联系,探讨自噬在SLE发病中的作用机制。结果 RT-q PCR显示:SLE患者外周血单个核细胞接受UVB照射后Uvrag,Beclin1和LC3ⅡmRNA的表达水平较照射前明显升高(P<0.05);SLE患者外周血单个核细胞接受UVB照射后LC3ⅡmRNA的表达水平明显高于正常对照组接受UVB照射的水平(P<0.01)。Western blotting显示:SLE患者的外周血单个核细胞接受UVB照射组较SLE患者组的外周血单个核细胞中的Uvrag,Beclin1和LC3Ⅱ的蛋白水平升高,Uvrag,Beclin1差异有统计学意义(P<0.05);SLE患者的外周血单个核细胞接受UVB照射组较正常人的外周血单个核细胞接受UVB照射组的Uvrag,Beclin1和LC3Ⅱ的蛋白水平轻度升高,LC3Ⅱ差异有统计学意义(P<0.05);SLE患者的外周血单个核细胞在接受UVB照射组较正常未照射UVB组的外周血单个核细胞中的Uvrag,Beclin1和LC3Ⅱ蛋白水平升高(P<0.05)。结论活动性SLE患者外周血单个核细胞中存在自噬现象,UVB能够引发SLE患者发生异常自噬,自噬相关基因/蛋白参与了SLE外周血单个核细胞异常自噬的发生。Objective To study the effects of UVB irradiation on autophagy in peripheral blood mononuclear cells of SLE patients,and to reveal the impacts of UVB irradiation and autophagy on SLE. Methods RT-qPCR and Western blotting techniques were used to measure the expression levels of the autophagy associated markers （Uvrag, Beclinl and LC3-II ）in peripheral mononuclear cells of patients with active SLE. Statistical analysis was performed to determine pathogenetic role of autophagy in active SLE. Results RT-qPCR results showed that the expression levels of LC3 II , Beclinl and Uvrag mRNA were significantly increased in active SLE patients after UVB-exposure compared to before UVB exposure （ P 〈 0. 05 ）. Following UVB irradiation, the expression levels of LC3 II mRNA were higher in SLE patients than in normal controls （ P 〈 0.01 ）. Moreover, UVB irradiation also significantly increased the expression levels of Beclinl and Uvrag proteins in SLE pa- tients （ P 〈 0. 05 ） while LC3 II expression was slightly increased. After UVB irradiation, the expression levels of LC3 II expression were significantly higher in SLE patients than in normal controls （ P 〈 0. 05 ）. In comparison with normal controls, the expression levels of LC3 II , Beclinl and Uvrag proteins were significantly higher in patients with active SLE after UVB exposure （P 〈 0.05 ）. Conclusion Autophagy occurs in peripheral blood mononuclear cells in patients with active SLE. UVB irradiation can alter autophagy in patients with active SLE. Autophagy related genes and associated proteins are likely involved in the process of autophagy occurring in peripheral blood mononuclear cells of patients with active SLE.

关 键 词：系统性红斑狼疮 UVB 自噬 单个核细胞 

分 类 号：R593.24[医药卫生—内科学]