ApoE^(-/-)小鼠的繁殖与鉴定  

Breeding and Genotype Identification of Apolipoprotein E Gene Knock Out Mice

作　　者：鲁晓丽[1] 杨文[1] 徐璐[2] 李红兵[1] 冉林武[1] 

机构地区：[1]宁夏医科大学实验动物中心,银川750004 [2]宁夏医科大学临床学院,银川750004

出　　处：《实验动物科学》2016年第1期29-32,37,共5页Laboratory Animal Science

基　　金：宁夏卫生计生重点研究课题(No.2013123;2015-NW-032);宁夏医科大学校课题(No.XT2011007;XM2015015)

摘　　要：目的繁殖和鉴定ApoE^(-/-)小鼠,建立血管损伤模型,为进一步研究动脉粥样硬化奠定基础。方法将引进的小鼠采用1只雌性与1只雄性同笼的方式进行繁殖。从子代小鼠尾巴中提取DNA,用PCR法和琼脂糖凝胶电泳法鉴定小鼠的基因型。获取ApoE^(-/-)纯合子小鼠,并绘制其0~20周龄生长曲线。采用油红O脂类染色法从血管形态学方面观察ApoE^(-/-)小鼠。结果成功繁育出的ApoE^(-/-)纯合小鼠较C57小鼠生长缓慢,且20周龄ApoE^(-/-)小鼠血管有明显损伤。结论利用ApoE^(-/-)纯合子小鼠能够建立良好的血管损伤模型。To breed and identify apolipoprotein E（ Apo E） gene knock out mice for further study of atherosclerosis with dyslipidemia model,apolipoprotein E- /-mice were bred and reproduced. Genomic DNA was isolated from tails and subjected to PCR and agarose gel electrophoresis for offspring genotype identification. The growth rate of ApoE-/-homozygous mice during 0—20 weeks of age were determined. The vascular morphological of ApoE-/-mice were observed by Oil Red O dye method. The results showed that ApoE-/-mice was successfully bred. The growth of ApoE-/-mice was slowly than wild type C57 BL /6 mice. And the obvious damage of vascular in ApoE-/-mice at 20- week age was obvious. Conclusion The damaged vascular model of ApoE-/-mice was successfully established.

关 键 词：载脂蛋白E-/-小鼠 基因型鉴定 

分 类 号：Q344[生物学—遗传学]