rpoD和gyrA基因对气单胞菌鉴定的比较分析  

Comparative analysis of rpo D and gyr A gene in identification of Aeromonas

作　　者：李欣悦[1] 李凤娟[1,2] 杜鹏程[1,3] 王瑞白[1] 王多春[1] 

机构地区：[1]中国疾病预防控制中心传染病预防控制所,北京102206 [2]河南省疾病预防控制中心,河南郑州450016 [3]北京市地坛医院,北京100011

出　　处：《疾病监测》2016年第3期200-204,共5页Disease Surveillance

基　　金：国家科技重大专项(No.2012ZX10004215)~~

摘　　要：目的使用rpo D和gyr A基因对气单胞菌进行种水平的鉴定,比较两者分类结果的差异,同时对本研究中187株气单胞菌进行较为准确的种水平的分类。方法对生化鉴定的187株气单胞菌进行rpo D和gyr A基因聚合酶链反应扩增并测序,将序列与种特异的参考序列一起构建系统发育树。结果 187株菌经rpo D基因鉴定为7个种,经gyr A基因鉴定为9个种,还有一个未定种。两者鉴定最多的3个种一致,即维氏气单胞菌温和生物变种、豚鼠气单胞菌和水族馆气单胞菌。gyr A基因鉴定增加的2个种是异嗜糖气单胞菌和简氏气单胞菌。rpo D和gyr A基因对气单胞菌鉴定一致的有174株(93%),但有13株菌(7%)两者鉴定不一致。结论 rpo D和gyr A基因能够对大部分气单胞菌进行准确一致的分类,对于两者鉴定不一致的菌株,应增加管家基因进行分析。Objective To identify Aeromonas at species level by using rpo D and gyr A gene,compare the identification results and get more accurate classifications of 187 strains in this study. Methods PCR amplification and sequencing were conducted for rpo D and gyr A gene of 187 strains of Aeromonas identified by biochemical method,the phylogenetic tree was constructed by using the sequencing results and the specific reference sequences. Results By rpo D gene sequence analysis,187 stains were classified into 7 species,however,by gyr A gene analysis,9 species were identified and 1 was unspecified,and the top three species were consistent with the results of rpo D gene analysis,namely,A. veronii bv. sobria,A. caviae and A. aquariorum. The two added species were A. allosaccharophila and A. jandaei. Among the Aeromonas strains identified,174（ 93%） had consistent identification results by using the two genes except 13 strains（ 7%）. Conclusion The detections of rpo D and gyr A genes can identify most Aeromonas strains,however,the detection of housekeeping genes is necessary to identify the strains which cannot be identified in rpo D and gyr A detection.

关 键 词：气单胞菌 rpoD基因 GYRA基因 鉴定 

分 类 号：R440[医药卫生—诊断学]