急性创伤性凝血障碍大鼠凝血因子Ⅶ的表达及其调节因素  

The expression and control factor of coagulation factor Ⅶ in rats with acute traumatic coagulopathy

作　　者：窦东伟[1] 薛寅凯 陈路佳[1] 朱鹏程[1] 孙运秀[2] 程平[1] 陈立波[1] 郑海[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院急诊创伤外科,武汉430022 [2]武汉市中心医院急诊科,430014

出　　处：《中华实验外科杂志》2016年第4期959-961,共3页Chinese Journal of Experimental Surgery

基　　金：基金项目：湖北省自然科学基金（2014CFB992）

摘　　要：目的探讨急性创伤性凝血障碍（ATC）大鼠体内凝血因子Ⅶ（FⅦ）的表达及其影响因素。方法建立大鼠ATC模型，监测大鼠平均动脉压（MAP）、凝血酶原时间（胛）和活化部分凝血活酶时间（APTT）的变化，检测大鼠FⅦ血液中的浓度以及在mRNA水平、蛋白质水平的表达，检测可能影响其表达的一氧化氮（NO）含量的变化。结果成功建立大鼠ATC模型，PT、APTF较空白对照组（Sham组）明显延长（P〈0．01）；ATC组大鼠血浆中FⅦ的浓度[（3．30±0．22）ng／ml]较Sham组[（5．35±0．10）ng/ml]明显降低（P〈0．01）；ATC组大鼠肝脏组织中FVIImRNA的相对表达水平明显降低（P〈0．01），Westernblot检测显示肝脏FⅦ蛋白的相对表达水平显著降低（P〈0．01）；ATC组大鼠血清NO含量[（4．96±0．26）μmol／L]与Sham组[（4．36±0．36）μmol／L]比较差异无统计学意义（P〉0．05），但肝脏中含量[（56．78±5．66）μmoL／kg]较Sham组[（35．07±3．85）μmol／kg]明显升高（P〈0．01）。结论FⅦ低表达可能是ATC发病的重因素，针对NO途径的靶向调控FⅦ的表达可能在治疗ATC中发挥作用。Objective To study the expression and control factor of coagulation factor Ⅶ （FⅦ） in rats with acute traumatic coagulopathy （ATC）. Methods ATC rats model was set up. The experimen- tal group rats were made to cause ATC, meanwhile, taking notes of changes of their mean arterial pressure （MAP）, prothrombin time （PT）, active partial thromboplastin time （APTT）, concentration of FⅦ, the level of mRNA, the expression of protein levels and nitric oxide （NO）. Results The ATC experimental group rats appeared prolonged ATC, PT and APTT compared with the control group（ P 〈 0.01 ）. The Ex- perimental group rats have lower concentration of FⅦ, which was （3.30 ± 0. 22 ） ng/ml while the control group was （5.35 ±0. 10） ng/ml （P 〈0. 01 ）. The FⅦ mRNA of the ATC group was reduced significantly in the liver tissue （P 〈 0. 01 ）. According to the result of Western blotting, Relative expression of the FⅦ protein expression of ATC group liver tissue decreased significantly （ P 〈 0. 01 ）. ATC group rats serum contains NO [ （4. 96 ±0, 26） μmol/L] and control group [ （4. 36 ±0. 36） μmol/L] there was negatively significant （P 〉0.05）, but the content in the liver [ （56. 78 ±5.66） μmol/kg] compared with the control group [ （35.07 ± 3.85 ） μmol/kg] increased significantly （ P 〈 0. 01 ）. Conclusion The low expression of FⅦ may be a major cause of ATC. Moreover, the method of targeting NO to regulate the expression of FⅦ may play an important role in the treatment of ATC.

关 键 词：急性创伤性凝血障碍 凝血因子Ⅶ 创伤性休克 一氧化氮 

分 类 号：R969.1[医药卫生—药理学]