沉默LASP-1对人胶质瘤细胞的黏附、迁移和侵袭能力的影响及其机制  

Effect and mechanism of LIM and SH3 protein 1 on adhesion, migration and invasion of human glioma cells

作　　者：倪洪早 张芸[1] 崔晨晨[1] 朱一硕 陈洪福[1] 范月超[1] 郑骏年[1] 

机构地区：[1]徐州医学院附属医院神经外科,221002

出　　处：《中华实验外科杂志》2016年第4期986-988,共3页Chinese Journal of Experimental Surgery

基　　金：基金项目：江苏省六大人才高峰基金（2014-WSW-033）

摘　　要：目的 探讨LASP-1基因在胶质瘤细胞迁移和侵袭中的作用及其机制.方法 运用Control小干扰RNA （siRNA）（Ctrl组）、LASP-1 siRNA（siRNA组）分别转染人脑胶质瘤细胞U251、U87,Western blot检测LASP-1、磷酸化黏着斑激酶（p-FAK）、Src、基质金属蛋白酶（MMP）-2和MMP-9蛋白表达量.检测沉默LASP-1基因对胶质瘤细胞黏附、迁移和侵袭能力的影响.结果 与Ctrl组比较siRNA组U87和U251细胞中LASP-1蛋白表达量降低;U87和U251黏附能力下降了59.14％和62.37％,迁移能力分别下降55.61％和56.07％,侵袭能力分别下降48.62％和56.31％.干扰LASP-1表达后siRNA组p-FAK、Src、MMP-2和MMP-9蛋白表达量较Ctrl组均明显降低,差异均有统计学意义（P＜0.05）.结论 干扰LASP-1基因通过p-FAK-Src信号通路,抑制MMP-2和MMP-9蛋白表达,从而抑制胶质瘤细胞的黏附、迁移和侵袭能力.Objective To investigate the effect and mechaIlism of LIM and SH3 protein 1 （LASP-1） on the adhesion,migration and invasion of human glioma cells.Methods The small interfering RNA （siRNA group） LASP-1 and Control siRNA （Ctrl group） were transfected into the U87 and U251 glioma cells using SilentFect Lipid Reagent.The expression of LASP-1 and the expression levels of phosphorylated focal adhesion kinase （p-FAK）,Src,matrix metalloproteinase-2 （MMP-2） and matrix metalloproteinase-9 （MMP-9） proteins after LASP-1 was konckdowned in both ofglioma cell lines were assessed by Western blotting.Results Compared to the Ctrl group,the siRNA group could decrease the expression of LASP-1 in both glioma cell lines significantly.The adhesion ability of U87 and U251 treatment with siRNA decreased by 59.14% and 62.37% and the cell migration ability reduced by 55.61% and 56.07% and the invasion ability was restrained by 48.62% and 56.31%.The expression of p-FAK,Src,MMP-2,MMP-9 and LASP-1 protein in siRNA group was significantly lower than that in Ctrl group.Conclusion Silencing LASP-1 gene inhibits the expression of MMP-2 and MMP-9 proteins through the p-FAK-Src pathway,which can inhibit the adhesion,migration and invasion of glioma cells.

关 键 词：LASP-1 胶质瘤 黏附 迁移 侵袭 

分 类 号：R329.25[医药卫生—人体解剖和组织胚胎学]