一氧化碳高压保存离体心脏的研究  

High pressure of carbon monoxide preservation on heterotopic transplantation of isolated heart

作　　者：黄成锋[1,2] 黄帅[1] 郑少忆[1] 卢聪[1] 郭惠明[1] 庄建[1] 朱平[1] 

机构地区：[1]广东省心血管病研究所广东省冠心病防治研究重点实验室广东省医学科学院广东省人民医院心脏外科,广州510080 [2]广州医科大学心胸外科,广州510925

出　　处：《中华实验外科杂志》2016年第4期1019-1022,共4页Chinese Journal of Experimental Surgery

基　　金：基金项目：中国科技部国际合作资助项目（2010DFA32660）;国家自然科学基金（81370230）;广东省自然科学基金（$2013010014009）

摘　　要：目的 探索一种全新的器官保存方法,并通过检测实验指标探讨其对器官离体保存的作用与机制.方法 以BALB/c 7周雄性小鼠为供受体,将供体心脏取出后,离体保存24 h,通过颈部异位移植移植到受体BALB/c 7周雄性小鼠中.实验分为3个实验组,分别为一氧化碳（CO）高压保存24 h移植组（CO组）;组氨酸-色氨酸-酮戊二酸盐液（HTK）浸泡保存24 h移植组（HTK组）;即刻移植组（正常组）.移植后记录心脏的复跳率;24h取异位心脏检测.检测心肌组织中血红素加氧酶-1（HO-1）及诱导型一氧化氮合酶（iNOs）含量,原位细胞凋亡检测（TUNEL）及羟基脱氧鸟苷（8-OHdG）检测.结果 Ho-1（ho-1/α-tubulin比值）：CO保存组（0.245）表达明显高于HTK保存移植组（0.168）和即刻移植组（0.058,P＜0.05）,而iNOs（即iNOS/α-tubulin值）的表达量最高是HTK保存移植组（0.268）,其次是CO保存移植组（0.124）及即刻移植组（0.082）.心肌凋亡百分比中,HTK保存移植组凋亡百分比明显高于CO保存移植、即刻移植两组,差异有统计学意义（P＜0.05）.结论 外源性的CO保存心脏,能通过有效的抗凋亡和减轻氧化损伤,减轻炎性反应等作用而对离体心脏起保护作用,而这种保护作用可能是通过调高心肌组织中的HO-1含量、抑制iNOs产生以及CO本身联合作用而形成.Objective To explore a new method of organ preservation,we assess the effect and mechanism of carbon monoxide （CO） on heart preservation.Methods BALB/c 7 week male mice were used to establish a model of mice cervical heterotopic heart transplantation.Mice were randomly divided into three groups：hearts preserved in CO gas combined with high pressure for 24 h group （CO group）,hearts immersed in HTK （histidine-tryptophan-ketoglutarate solution） for 24 h group （HTK group）,and immediate transplantation group （control group）.In CO and HTK groups,hearts were transplanted after 24 h preservation.The re-beating time and state of the transplanted hearts were recorded.Heart tissues were collected after 24 h transplantation.Levels of Human heme oxygenase-1 （HO-1）,inducible nitric oxide synthase （iNOs）,and 8-Hydroxy-desoxyguanosine （8-OHdG） were measured.Cell apoptosis of transplanted hearts were assessed by tunel staining.Results The HO-1 expression levels in CO group （0.245） were significantly higher than HTK group （0.168,P 〈 0.05） and control group （0.058,P 〈 0.05）.While the iNOs expression levels of HTK group （0.268） were higher than CO group （0.124） and control group （0.082）.The apoptosis index of HTK group was significantly higher than CO group and control group （P 〈 0.05）.Conclusion Exogenous CO can inhibit apoptosis,oxidative damage,and inflammation of donor hearts probably via increasing HO-1 and reducing iNOs.

关 键 词：一氧化碳 心脏保存 

分 类 号：R457.1[医药卫生—治疗学]