大鼠单侧输尿管梗阻24h内肾脏水通道蛋白2表达变化  

Time course of downregulation of aquaporin 2 in response to unilateral ureteral obstruction in rats

作　　者：车英玉[1] 张鹏[3] 王焱[2] 王庆伟[2] 文建国[2] 付汪星[1] 

机构地区：[1]郑州大学第一附属医院磁共振科,450052 [2]河南中医药大学第二临床医学院,郑州450002 [3]郑州大学第一附属医院泌尿外科,450052

出　　处：《中华实验外科杂志》2016年第4期1041-1043,共3页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金（81370869）

摘　　要：目的 观察大鼠单侧输尿管梗阻（UUO） 24 h内肾脏水通道蛋白2（AQP2）表达变化及其对肾脏功能的影响.方法 28只慕尼黑大鼠行单侧输尿管结扎术,分别于术后2h（UUO-2组）、6h（UUO-6组）、12h（UUO-12组）、24h（UUO-24组）收集血液和肾脏标本（n=7）,检测血浆电解质和渗透压,Western blot检测肾脏AQP2蛋白水平.各组均设等量（n=7）假手术对照组（Sham-2、Sham-6、Sham-12、Sham-24组）.结果 与Sham组比较,UUO-24 h血浆渗透压升高[（347.6±1.7）比（304.3±1.3） mosmol/kg H2O],UUO-2 h血浆肌酐[（39.6±5.8）比（10.5±4.3）μmol/L]、尿素[（9.2±0.4）比（6.9±0.3） mmol/L]和血钾[（5.3±0.2）比（4.3 ±0.1）mmol/L]升高,UUO-2、UUO-6、UUO-12组,24h血浆肌酐呈上升趋势.UUO-6、UUO-12组,24h血钠水平显著低于Sham组[（135.6±1.3）比（137.4±0.8）,（126.3±1.3）比（136.5±1.2）,（135.2±1.0）比（139.7±1.5） mmol/L].Western blot显示UUO-12 h和UUO-24 h导致AQP2在肾脏内髓表达分别下降到Sham组的69％和22％,各组比较差异有统计学意义（P＜0.05）.结论 单侧输尿管梗阻后12h开始出现肾脏AQP2蛋白表达显著下降,肾脏功能改变早于AQP2蛋白变化.Objective To investigate the early time course of dysregulation of aquaporin 2 （AQP2） and renal function after 2-,6-,12-,24-h unilateral ureteral obstruction （UUO）.Methods Twenty-eight Munich-Wistar rats were subjected to UUO,and at 2 h （UUO-2,n =7）,6 h （UUO-6,n =7）,12 h （UUO-12,n =7）,and 24 h （UUO-24,n =7）,blood and kidney samples were collected.Age-and time-matched sham-operated controls （n =7） were prepared and observed in parallel for each UUO group.Between 2 and 24 h,osmolality and electrolyte concentrations in plasma were determined.The dysregulation of AQP2 was detected by by semiquantitative immunoblottling.Results As compared with sham group,2-h UUO resulted in a significant increases in the level of plasma creatinine [（39.6±5.8） vs.（10.5±4.3） μmol/L],urea [（9.2±0.4） vs.（6.9±0.3） mmol/L],and serum potassium [（5.3 ±0.2） vs.（4.3 ±0.1） mmol/L].Rats subjected to 6-,12-and 24-h UUO had decreased plasma sodium levels [（135.6 ± 1.3） vs.（137.4 ± 0.8） mmol/L,（126.3 ± 1.3） vs.（136.5 ±1.2） mmol/L,and （135.2 ± 1.0） vs.（139.7 ± 1.5） mmol/L,respectively] as compared with sham group.Plasma osmolality was higher after 24-h UUO than in sham group [（347.6 ± 1.7） vs.（304.3 ± 1.3） mosmoL/kg H2O].The AQP2 protein abundance did not change in the kidneys from 2-and 6-h UUO rats,but after 12-and 24-h UUO the significant down-regulation was observed （69% and 22%,respectively） as compared with sham group.Conclusion AQP2 protein abundance did not change in obstructed kidneys from 2-and 6-h UUO rats,but is significantly down-regulated after 12-and 24-h UUO.The damage of renal function observed is earlier than the down-regulation of AQP2 protein.

关 键 词：单侧输尿管梗阻 水通道蛋白2 肾脏功能 

分 类 号：R693.2[医药卫生—泌尿科学]