普罗布考对动脉粥样硬化兔的高密度脂蛋白逆转运功能中酶蛋白和受体的影响  

Effect of Probucol on Enzyme and Receptors of High Density Lipoprotein During Reverse Cholesterol Transportation in Experimental Rabbits With Atherosclerosis

作　　者：钟建开[1] 吴焱贤[1] 陈盈文[1] 李琛[2] 黎文生[1] 郭志刚[2] 

机构地区：[1]佛山市顺德第一人民医院心内科,广东省528300 [2]南方医科大学附属南方医院心内科

出　　处：《中国循环杂志》2016年第4期393-397,共5页Chinese Circulation Journal

基　　金：广东省医学科学技术研究基金(B2014388)

摘　　要：目的:探讨普罗布考对动脉粥样硬化(AS)兔的高密度脂蛋白逆转运功能中酶蛋白和受体的影响。方法:新西兰大白兔(共24只)随机分为3组:对照组(n=8):用普通饲料饲养;高脂组(n=8):用高脂饲料喂养;普罗布考组(n=8):在高脂饲料的基础上给予普罗布考喂养。饲养12周通过比色法测定血脂,通过酶联免疫吸附法检测血清磷脂胆固醇酰基转移酶(LCAT)和胆固醇酯转移蛋白(CETP),并采用免疫组化方法检测主动脉壁斑块内三磷酸腺苷结合盒转运体A1(ABCA1)和清道夫受体-BI(SR-BI)表达水平。结果:实验后第12周(1)血脂指标:与高脂组比较,普罗布考组血清总胆固醇[TC,(15.95±1.51)mmol/L vs(21.95±3.71)mmol/L]、低密度脂蛋白胆固醇[LDL-C,(13.01±2.28)mmol/L vs(17.90±3.51)mmol/L]、高密度脂蛋白胆固醇[HDL-C,(0.56±0.10)mmol/L vs(1.13±0.12)mmol/L]水平明显下降,差异均有统计学意义(P<0.01)。(2)LCAT和CETP:与对照组[CETP(2.07±0.64)μg/ml和LCAT(27.01±8.26)μg/ml]比较,高脂组[CETP(1.24±0.54)μg/ml和LCAT(15.02±3.81)μg/ml]明显降低,差异均有统计学意义(P<0.05);与高脂组比较,普罗布考组[CETP(3.43±1.01)μg/ml和LCAT(38.10±7.96)μg/ml]明显升高,差异均有统计学意义(P<0.05)。(3)主动脉壁斑块ABCA1和SR-BI受体表达:与高脂组比较,普罗布考组ABCA1阳性表达的面积(%)显著增加[(46.81±10.01)%vs(24.10±8.48)%,P<0.01];SR-B1阳性表达面积(%)亦显著增加[(48.04±10.90)%vs(18.61±6.77)%,P<0.01],两组比较差异均有统计学意义。结论:普罗布考通过增加高密度脂蛋白外周动脉受体ABCA1和SR-BI表达,增加LCAT和CETP的量,促进HDL的RCT功能,从而改善HDL的功能。Objective： To explore the effect of probucol on enzyme and receptors of high density lipoprotein（HDL） during reverse cholesterol transportation in experimental rabbits with atherosclerosis. Methods： A total of 24 New Zealand rabbits were randomly divided into 3 groups： Control group,the rabbits were fed by normal diet,High cholesterol group,the rabbits were fed by high cholesterol diet and Probucol group,the rabbits were fed by high cholesterol diet with probucol. n=8 in each group,all animals were treated for 12 weeks. Blood levels of lipids were examined by colorimetric method,serum lecithin cholesterol acyltransferase（LCAT） and cholesterol ester transfer protein（CETP） were detected by ELISA,expressions of ATP binding cassette transporter A1（ABCA1） and scavenger receptor class B type I（SR-BI） in aortic plaque were measured by immunohistochemistry; the above indexes were compared among different groups.Results：①for blood lipids by mmol/L,the following indexes in Probucol group were lower than High cholesterol group： TC（15.95±1.51 vs 21.95±3.71）,LDL-C（13.01±2.28 vs 17.90±3.51）,HDL-C（0.56±0.10 vs 1.13±0.12）,all P0.01.②the following perimeters in High cholesterol group were lower than Control group： for CETP by μg/ml（1.24±0.54 vs 2.07±0.64）,for LCAT by μg/ml（15.02±3.81 vs 27.01±8.26）,all P0.05; compared with High cholesterol group,Probucol group had increased CETP（3.43±1.01） and LCAT（38.10±7.96）,all P0.05.③positive expressions of ABCA1 and SR-BI in aortic plaque in Probucol group were higher than High cholesterol group： for ABCA1 by %（46.81±10.01 vs 24.10±8.48）,for SR-BI by %（48.04±10.90 vs 18.61±6.77）,all P0.01.Conclusion： Probucol may increase blood levels of LCAT,CETP via up-regulating the expressions of ABCA1,SR-B1 and elevating the reverse cholesterol transportation of HDL,therefore improve HDL function in experimental rabbits with atherosclerosis.

关 键 词：动脉粥样硬化 普罗布考 脂蛋白类 HDL 

分 类 号：R541[医药卫生—心血管疾病]