基于16S rRNA序列分析南糯山不同生境茶树根际土壤细菌群落分布多样性  

Bacteria Distribution Diversity in Tea Rhizospheric Soil from Different Habitats at Nannuo Mountain Based on 16S rRNA Sequence Analysis

作　　者：卢开阳[1,2,3] 张云峰[1] 贾鳗 高林瑞 田飞[2,3,4] 唐蜀昆[2,3,4] 

机构地区：[1]云南师范大学生命科学学院,云南昆明650500 [2]大益集团微生物研发中心,云南昆明650200 [3]云南省普洱茶发酵工程研究中心,云南昆明650200 [4]云南大学云南省微生物研究所,云南昆明650091

出　　处：《昆明理工大学学报（自然科学版）》2016年第2期89-95,共7页Journal of Kunming University of Science and Technology(Natural Science)

基　　金：国家自然科学基金项目(31160003)

摘　　要：采用平板涂布分离法,探究南糯山古茶树和台地茶根际土壤细菌群落结构的多样性和差异性.针对不同种植环境下的大叶种茶树根际土样20份,采用6种培养基分离纯化共得到800株菌,通过形态学特征去重复后,得到250株代表菌株进行16S rRNA基因序列分析鉴定,共分布于17个科26个属,分属于放线菌门(Actinobacteria),厚壁菌门(Firmicutes)和变形菌门(Proteobacteria).其中古茶树生境分离到20个属,台地茶生境分离到17个属,在属一级水平,古茶树生境具有更高的多样性.两生境共有类群在属一级有11个,占42%.基于属间相对分离频率(RF)的t检验P=0.03(<0.05),不同生境下的细菌群落组成差异性显著,而丰富的链霉菌(Streptomyces)类群则是该区域茶树根际细菌群落组成的一个显著特征.This paper explores the bacterial community diversity and species composition differences in root soil of ancient and tableland tea tree by dilution coated plate method. 10 root soil samples are collected respectively from ancient and tableland tea tree in Nannuo Mountain,which are then isolated with dilution plate cultivation.About 800 strains are gained,250 of which are chosen after observation of their morphological characteristics to analyze the 16 S rRNA gene sequence. The strains belong to 26 genera,which are distributed in Actinobacteria,Firmicutes and Proteobacteria. The ancient tea tree root soil environment includes 20 genera while tableland has17 genera. There are 11 common genera in both habitats,accounting for 42%. Ecology diversity index analysis and t-test P = 0. 03（ 0. 05） show that these two habitats have obvious differences in bacterial community composition. The dominant bacteria species is Streptomyces in both habitats.

关 键 词：大叶种茶树 根际土壤 细菌群落多样性 

分 类 号：Q938.1[生物学—微生物学]