年轻红细胞制备及其平均年龄评价  

Preparation of Young Erythrocytes and Evaluation of Their Mean Cell Age

作　　者：韩玮[1] 刘景汉[1] 骆群[1] 欧阳锡林[1] 王海宝[1] 

机构地区：[1]解放军总医院输血科,北京100853

出　　处：《中国医师杂志》2002年第8期840-842,共3页Journal of Chinese Physician

基　　金：基金课题 :"九.五"军队医药卫生科研基金重点课题 ;编号 :98Z0 86

摘　　要：目的　建立一种简便易行的制备年轻红细胞的方法 ,确定并验证评价平均红细胞年龄的指标。方法　选取 34名健康献血者 ,各采集 4 0 0ml全血 ,滤除白细胞后使用普通输血用离心机和血袋 ,通过 2次离心并手工分离 ,取上层的红细胞即获得年轻红细胞。对分离前红细胞、年轻红细胞和老年红细胞 ,分别测定丙酮酸激酶(PK)活性、网织红细胞计数 (REI)、红细胞平均容积 (MCV)、红细胞平均血红蛋白浓度 (MCHC)、红细胞变形指数(EI)等相关指标并测定血红蛋白 (Hb)回收率。结果　上述各相关指标在三种红细胞间的差值均有显著意义。分得的年轻红细胞与分离前红细胞PK活性比率为 (1 175± 0 13) ,评估的平均红细胞年龄约为 4 0d ;血红蛋白回收率为 (48 37± 3 82 ) %。结论　该方法能够有效地制备年轻红细胞。PK、REI、MCV、MCHC、EI可以作为评价平均红细胞年龄的指标。Objective To establish a simple and feasible method of preparing young erythrocytes and test some indexes of evaluating the mean cell age.Methods We collected 34 bags of whole blood from healthy blood donors,400ml/each bag,then eliminated WBCs by leukoreduction filter.After twice centrifuging and manual speration,we finally obtained young erythrocytes from the upper section of the red cell layer,the remains were old erythrocytes.These different age erythrocytes were separated by blood bags.Some indexes as pyruvate kinase,reticulocyte count,mean corpuscular volume(MCV),mean corpuscular hemoglobin concentraion(MCHC),erythrocytes deformation index EI were tested on the non-separation erythrocytes,young erythrocytes and old erythrocytes,simultaneouosly,the detecting hemoglobin recovery rate.Results There was significant difference of all indexes among the three kinds of erythrocyte products.The PK activity ratio of young erythrocytes' to non-preparating erythrocytes was 1 175±0 13,showed the mean cell age was about 40 days.The hemoglobin recovery rate was(48 37±3 82)%.Conclusions The method is effective for preparing young erythrocytes,PK,REI,MCV,MCHC and EI can be used to evaluate the young erythrocytes' mean cell age.

关 键 词：制备 平均年龄 年轻红细胞 丙酮酸激酶 网织红细胞 白细胞过滤 

分 类 号：R457[医药卫生—治疗学]