不同部位取样对鸡基因组DNA提取的效果的影响  

Effect on DNA isolation from different samples of the chicken

作　　者：李龙飞[1] 汉丽梅[1] 李一帆[1] T. Rabie 

机构地区：[1]沈阳农业大学,辽宁沈阳110161 [2]Suez Canal University,Faculty of Agriculture Ismailia, Egypt

出　　处：《饲料工业》2016年第8期55-57,共3页Feed Industry

基　　金：辽宁省百千万人才工程培养经费资助[2012921027]

摘　　要：采用伤害性取样和非伤害性取样,采集鸡的肝脏、血液、羽髓、羽鞘、羽根、实心羽茎和羽毛上脐周围绒羽等不同部位样品,并采取对样品不同处理程序提取基因组DNA。结果表明,鸡的微量血液(50μl)和羽髓样品提取基因组DNA与肝脏(50 mg)样品提取的基因组DNA的量相当;羽鞘、羽根、实心羽茎和羽毛上脐周围绒羽可以提出一定量的基因组DNA,其提取量少于肝脏和羽髓组织,但可以满足各种涉及PCR反应的分子生物学试验,肝脏羽髓消化最适时间为2.5 h,其他样品需消化过夜。该研究结果为禽类分子生物学研究拓宽了样本来源。Liver, blood, feather pulp, theca of feather, quill, solid rhaehis, and plumila of upper umbili- cusand different parts of feather samples of chicken were sampled by destructive and nondestructive sampling for the DNA isolation. The different treating protocols were used for the isolation. The results showed that the yields and qualities of DNA isolated from blood （50μl） and feather pulp are nearly to livers＇, and those from theca of feather, quill, solid rhachis and plumila of upper umbilicus are some less but it is suitable to the experiment of molecular biology involved PCR. The protocol of DNA isola- tion provides new sources of samples for ornithic molecular biology study.

关 键 词：鸡 DNA提取 伤害性取样 非伤害性取样 

分 类 号：S852[农业科学—基础兽医学]