合成肽检测巨细胞病毒酶联免疫方法的建立及应用  

Establishment of ELISA Method of Detecting Human Cytomegalovirus Using Synthetic Peptide and its Application

作　　者：卢银平[1] 董继华[1] 曹伟[2] 熊腊香[2] 

机构地区：[1]华中科技大学同济医学院附属协和医院病毒室,湖北武汉430022 [2]华中科技大学同济医学院附属协和医院妇产科

出　　处：《中国医师杂志》2002年第8期852-854,共3页Journal of Chinese Physician

摘　　要：目的　应用合成肽建立检测人巨细胞病毒 (HCMV)特异敏感的酶联免疫方法 ,并用于临床检测患者血清中HCMV -IgM。方法　根据人巨细胞病毒PP15 0氨基酸序列 ,利用计算机辅助设计 ,决定含HCMVPP15 0较强抗原决定簇的单链多肽序列 ,合成肽作抗原 ,建立检测抗HCMV -IgM的酶联免疫吸附试验 (ELISA)法 ,并用聚合酶链反应 (PCR)方法检测全血中HCMVDNA ,比较ELISA与PCR两种方法的相关性。结果　本实验所建立的检测抗HCMV -IgM的ELISA方法具有较好的特异性 ,正常孕妇血清中抗HCMV -IgM阳性率与HCMVDNA存在相关性。结论　本研究所建立的以合成多肽作抗原检测巨细胞病毒酶联免疫方法具有较好的特异性 ,简单、快速 ,适用与临床标本的检测。Objective To establish the ELISA method by using synthetic peptide for detection of IgM antibodies to human cytomegalovirus(HCMV).Methods According to HCMV PP150 sequence of amino acid and nucleotide,the peptide sequence was decided by computer with some program of software of protein.The ELISA method using synthetic peptide as antigen was developed and was applied to detected anti-HCMV IgM in sera of normal pregnant woman and habitual abortion woman.The HCMV DNA were detected by polymerase chain reaction(PCR).Results The positive rates of anti-HCMV IgM in different populations were as follows:9 17%(11/120) in normal pregnant woman,37 5%(24/64) in habitual abortion woman.The ELISA method have good speciality.The positive rate of HCMV DNA was significantly related to that of anti-HCMV IgM(P<0 005).Conclusions This ELISA method using synthetic peptide as antigen was special,sensitive,simple and quick,it can be applied to detect HCMV IgM in sera of patients with active infection of HCMV.

关 键 词：人巨细胞病毒 酶免疫吸附试验 合成肽 HCMV 

分 类 号：R446.61[医药卫生—诊断学]