Caspase-3及Cleaved-Caspase-3在氟中毒大鼠肝脏中表达  

The expression of Caspase-3 and Cleaved-Caspase-3 in liver of the fluorosis rats

作　　者：李永宁[1] 肖晶晶[1] 赵建勇[1] 

机构地区：[1]贵州医科大学,贵州贵阳550004

出　　处：《贵州医药》2016年第3期239-240,F0003,共3页Guizhou Medical Journal

基　　金：贵阳市科学技术计划项目上[(2010)筑科农合字第1-社-19号]

摘　　要：目的探讨氟中毒大鼠肝脏组织中Caspase-3及Cleaved-Caspase-3的表达情况。方法选用36只健康SD大鼠分为对照组、低氟组、高氟组(饮用含氟量分别为<1mg/L、5mg/L及50mg/L的自来水),每组12只(雌雄各半)。饲养6个月后,股动脉放血处死,氟离子电极法检测大鼠尿氟含量;自动血生化分析仪检测大鼠肝功能;免疫组织化学法和蛋白印记(Western blot)法检测Caspase-3及Cleaved-Caspase-3蛋白表达水平。结果低氟组、高氟组大鼠尿氟[(1.85±0.13)、(2.23±0.10)mg/L均高于对照组[(1.63±0.11)mg/L](P<0.05);低、高氟组大鼠血清谷丙转氨酶及谷草转氨酶活性[(48.66±5.55)、(68.17±8.39)U/L及(142.57±16.21)、(165.89±28.26)U/L]均高于对照组[(38.49±2.98)、(117.98±9.88)U/L](P<0.05);高过剂量氟组Caspase-3及Cleaved-Caspase-3蛋白表达[(98.08±13.08)、(106.33±9.14)]明显高于对照组[(53.92±6.36)、(59.78±7.54)](P<0.05)。结论氟可促进Caspase-3及Cleaved-Caspase-3蛋白的表达,其可能参与慢性氟中毒所致肝脏损伤发病过程。Objective To explore the role of Caspase-3and Cleaved-Caspase-3in rats liver damaged by excessive fluorine.Methods Thirty-six healthy Sprague-Dawley（SD）rats were randomly divided into three groups which were control group（drunk water containing NaF1mg/L）,low and high fluorosis groups（drunk water containing NaF of 5mg/L and 50mg/L）.After 6months of experiment treatment,the fluorine contents of urine were detected by fluorin-ion electrode method.The rats liver function determined by automatic blood chemical analyzer.The protein expression of Caspase-3and Cleaved-Caspase-3were detected by immunohistochemistry（IHC）and protein imprinting（western blot）.Results The fluorine contents of urine in low and high-dose fluoride groups [（1.85±0.13）,（2.23±0.10）mg/L]was higher than control group [（1.63±0.11）mg/L]（P〈0.05）.The activity of serum ALT and AST in low and high-dose fluorosis group[（48.66±5.55）,（68.17±8.39）U/L and（142.57±16.21）,（165.89±28.26）U/L]was higher than control group[（38.49±2.98）,（117.98±9.88）U/L]（P〈0.05）.The protein expression of Caspase-3and Cleaved-Caspase-3 [（98.08±13.08）,（106.3±9.14）]in high-dose fluorosis group were significantly increased than control group [（53.92±6.36）,（59.78±7.54）]（P〈0.05）.Conclusion Caspase-3and Cleaved-Caspase-3may become the pathogenesis of liver damage in chronic fluorosis.

关 键 词：氟中毒 CASPASE-3 Cleaved-Caspase-3 细胞凋 

分 类 号：R392.11[医药卫生—免疫学]