^(60)Co-γ辐射对桂花种子萌发及幼苗生长的影响  

Study on the Seed Germination and Seedling Growth of Osmanthus fragrans Under ^(60)Co- γ Irradiation

作　　者：耿兴敏[1] 王良桂[1] 李娜[1] 杨秀莲[1] 

机构地区：[1]南京林业大学风景园林学院,江苏南京210037

出　　处：《核农学报》2016年第2期216-223,共8页Journal of Nuclear Agricultural Sciences

基　　金：江苏省高校优势学科建设工程资助项目(PAPD);"十二五"科技支撑林木种质资源发掘与创新利用(2013BA001B)

摘　　要：为探讨辐射对桂花种子的诱变作用,以不同剂量的60Co-γ射线对7个桂花品种的种子进行辐射处理,观察其对桂花种子萌发及出苗状况的影响。结果表明,辐射处理对桂花种子萌发状况的影响存在显著的品种间差异。除籽银桂外,其它6个品种种子发芽率均与辐射剂量呈负相关,随着辐射剂量的增加,6个桂花种子的相对发芽率和出苗率明显降低。6个桂花种子的半致死剂量大小顺序依次为潢川金桂>紫梗籽银桂>短柄籽银桂>米花籽银桂>金盏碧珠>宽叶籽银桂,分别为267、151、146、135、98和55Gy。低剂量(50Gy)的辐射能显著提高潢川金桂、短柄籽银桂和金盏碧珠3个品种种子的萌发率及出苗率;高剂量(200Gy)辐射后种子虽能萌发,但出苗率受到明显的抑制,尤其是250Gy时,除了不受辐射剂量影响的籽银桂外,其余6个品种出苗率均为0。本研究结果可为桂花辐射育种提供依据,促进桂花育种进程。Traditional Cross breeding of Osmanthus fragrans is very difficult due to small size of flowers,styles,and very few of pollens. To investigate the effects of irradiation on Osmanthus fragrans seeds,seven varieties of Osmanthus fragrans were irradiated with different radiation doses of ^60Co-γ,and the effects of radiation on seed germination and seedling growth were observed. The results indicated that the effects of radiation on seed germination varied among different varieties. Except for Zi Yingui,seed germination rate of the other six varieties was negatively correlated with radiation doses,the rate of germination and seedling were decreased with the increase of doses. Semi- lethal dose of six varieties of Osmanthus fragrans was 267,151,146,135,98 and 55 Gy for Huangchuang jingui,Zhigeng zi yingui,Duanbing Zi yingui,Mihua Zi yingui,Jinzhan bzhu and Kuanye zi yingui respectively. Lower dose（ 50Gy） of radiation increased the germination of Huangchuan Jingui,Duanbing Zi Yin and Jinzhan bizhu. With high dose（ 200Gy）,the rate of germination and seedling were inhibited greatly,especially with 250 Gy,all materials except Zi yingui,the others cannot germinate. The results will provide a theory basis for mutation breeding and accelerate the breeding process of Osmanthus fragrans.

关 键 词：^60Co-γ 桂花 诱变育种 种子萌发 幼苗生长 

分 类 号：S685.13[农业科学—观赏园艺]