兰花杂交育种中杂种F_1的早期鉴定  

Early Identification of Hybrid F_1 in Cross Breeding of Cymbidium

作　　者：李玉萍[1] 罗凤霞[1] 汤庚国[2] 史慧梅[1] 

机构地区：[1]金陵科技学院园艺学院,江苏南京210038 [2]南京林业大学森林资源与环境学院,江苏南京210037

出　　处：《核农学报》2016年第4期676-684,共9页Journal of Nuclear Agricultural Sciences

基　　金：江苏省农业科技自主创新基金[CX(12)2021];国家林业局公益性专项(201304117)

摘　　要：为了更好地在兰花育种中判别真杂种,采用SRAP分子标记技术对春兰紫萼×大花蕙兰日本绿的10个杂种F1株系进行杂种真实性鉴定。结果表明,21对多态性较高的随机引物共扩增得到364条带,多态性条带达307条,占总带数的84.34%,试验所选10个杂种F1株系均为真实杂种,且与母本相似性较高。聚类分析表明,SRAP分子标记技术可作为兰花杂交育种早期杂交种筛选鉴定的有效方法。因此,利用SRAP分子标记技术可为兰花育种筛选提供理论依据,加快兰花育种进程。In order to distinguish the hybrid in orchid cross breeding,SRAP molecular markers were used to identify hybrid reliability of 10 hybrid lines among Cymbidium goeringii Zi＇e × C.hybridum Riben LYu.The results suggested that 364 DNA bands were obtained with 21 pairs of highly polymorphic random primers.Among these DNA bands amplified,307（ 84.34%） were polymorphic.Hybrid Identification results showed that the selected 10 hybrid F1 were true hybrids with high similarity to their mothers.Clustering analysis showed that SRAP molecular markers are effective in identifying and screening early hybrids in the orchid cross breeding.Therefore,using SRAP molecular marker technology can provide a theoretical basis for screening orchid hybrid and orchid accelerate the breeding process.

关 键 词：兰花 杂交育种 SRAP F1鉴定 

分 类 号：S682.31[农业科学—观赏园艺]