栝楼雄株茎叶酚酸类化学成分研究  

Phenolic Acid in Stems and Leaves of Male Plants in Trichosanthes kirilowii Maxim.

作　　者：刘飞[1] 方磊[2] 李佳[1] 张永清[1] 

机构地区：[1]山东中医药大学,山东济南250355 [2]山东省分析测试中心,山东济南250014

出　　处：《中国现代中药》2016年第4期407-409,共3页Modern Chinese Medicine

基　　金：国家科技支撑计划(2011BAI06B06);山东省科技发展计划项目(2011GSF11904);公益性行业科研专项(201407002)

摘　　要：目的:研究栝楼雄株茎叶中的酚酸类成分,充分利用生产中的废弃资源。方法:乙醇提取,提取物以适量水混悬,依次采用石油醚、乙酸乙酯、正丁醇萃取,利用聚酰胺和Sephadex LH-20柱色谱、C_(18)中压柱色谱及制备型高效液相色谱等手段进行分离纯化,根据各化合物的理化性质及谱学数据进行结构鉴定。结果:共分离得到8个酚酸类化合物,分别鉴定为反式桂皮酸(1)、香草酸(2)、反式阿魏酸(3)、3-羟基乙酰基吲哚(4)、对羟基苯甲酸(5)、对羟基桂皮酸(6)、原儿茶酸(7)、对羟基苯甲醛(8)。结论:除化合物5以外,其他化合物均为首次从该部位分离得到,化合物1、3、4、6、7均为首次从该植物中分离得到,为生产中废弃资源的综合利用提供了参考。Objective： To study the phenolic acid in stems and leaves of male plants in Trichosanthes kirilowii Maxim. and make full use of waste resources in production. Methods： The plant material was extracted with ethanol. The ethanol extract was suspended in a moderate amount of water, extracted successively with petroleum ether, ethyl acetate and n- butanol, and compounds were separated and purified by polyamide resin, Sephadex LH-20, C18 middle-pressure chromatography and semi-preparative high performance liquid chromatography. The chemical structures of the compounds were determined on the basis of the chemical properties and spectral analysis. Results： Eight phenolic acids were isolated from stems and leaves of male T. Kirilowii, which were identified as trans-cinnamic acid （ 1 ）, vanillic acid （2）, trans-ferulic acid （3）, 3-（hydroxyacetyl） indole （ 4 ）, p-hydroxybenzoic acid （ 5 ）, p-Hydroxycinnamic acid （ 6 ）, protocatechuic acid （ 7 ）, and p- hydroxybenzaldehyde（8）. Conclusion： All compounds except compound 5 were isolated from this part for the first time, and compounds 1, 3, 4, 6, and 7 were isolated from this plant for the first time. It provides a reference for the comprehensive utilization of waste resources in production.

关 键 词：栝楼 雄株茎叶 酚酸类 提取分离 结构鉴定 

分 类 号：R917[医药卫生—药物分析学]