盾叶薯蓣药材质量标准研究  

Study on Quality Standards of Dioscoreae zingiberensis Rhizoma

作　　者：康阿龙[1] 汤迎爽[2] 孙文基[3] 

机构地区：[1]解放军第451医院,陕西西安710054 [2]解放军第323医院,陕西西安710054 [3]西北大学陕西省生物医药重点实验室,陕西西安710069

出　　处：《中国现代中药》2016年第4期448-451,共4页Modern Chinese Medicine

基　　金：陕西省中药材标准项目(2010-038)

摘　　要：目的：建立盾叶薯蓣药材的质量标准，为该药材及其制剂的质量控制提供有效方法。方法：采用显微和薄层色谱法鉴别，并对10批药材的水分、浸出物进行检查，采用HPLC对药材中薯蓣皂苷元含量进行了测定。结果：确定了盾叶薯蓣药材的显微特征，建立了TLC鉴别方法，拟定了其水分、浸出物限量。薯蓣皂苷元的进样量在2．36～21．24μg线性关系良好（r=0．9999），平均回收率为97．33％，RSD=1．28％（n=6）。结论：该方法操作简单、重现性好、准确可靠，可较全面地控制盾叶薯蓣药材及其制剂的质量。Objective： To establish the quality standard of Dioscoreae zingiberensis Rhizoma for providing the effective quality control method of the herb and its preparations. Methods ： 10 batch the plants were identified by microscopic and TLC methods, the contents of extract and water were determined. The content of diosgenin in Huangjiang was determined by HPLC. Results： The microscopic characteristics of Dioscoreae zingiberensis Rhizoma had been detected. TLC identification method had been established. The content of moisture and extract had been measured. Diosgenin showed a good linear relationship within the range of 2. 36-21.24 μg（r =0. 999 9）, The average recovery was 97. 33%, and the RSD was 1.28% （n = 6 ）. Conclusion： This method is simple, accurate and with good reproducibility, particularly suitable for the comprehensive quality control of Dioscoreae zingiberensis Rhizoma and its preparations.

关 键 词：盾叶薯蓣 质量标准 TLC HPLC 检查项 薯蓣皂苷元 

分 类 号：R282.71[医药卫生—中药学]