丹蒌片联合瑞舒伐他汀对球囊损伤内皮诱导的兔腹主动脉粥样硬化的影响  

Prevention Effect of Danlou Pills and Rosuvastatin on Atherosclerosis in Rabbit Atherosclerosis Model with Catheter-Induced Abdominal Aortic Wall Injury

作　　者：王贺[1] 司春婴[1] 罗明华[1] 解金红[1] 陈玉善[1] 关怀敏[1] 

机构地区：[1]河南中医学院第一附属医院,河南郑州450000

出　　处：《辽宁中医杂志》2016年第4期840-843,I0005,共5页Liaoning Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(81273949/H2902);河南省教育厅项目(14A320051)

摘　　要：目的:探讨丹蒌片联合瑞舒伐他汀抗动脉粥样硬化的作用及机制。方法:雄性日本大耳白兔40只,随机分为正常对照组(CL组)、模型组(M组)、丹蒌片组(DL组)、瑞舒伐他汀组(RS组)、丹蒌片联合瑞舒伐他汀组(联合治疗组,IT组),每组8只。正常对照组饲喂普通饲料,余各组腹主动脉球囊损伤后高脂饲料喂养制备动脉粥样硬化模型;RS组[1 mg/(kg·d)]、DL组[0.5 g/(kg·d)]及IT组[0.5 g/(kg·d)]加[1 mg/(kg·d)]除高脂饲料喂养外喂饲相应药物。连续给药9周后处死,测各组血脂、一氧化氮(NO)和一氧化氮合酶(e NOS)的含量;HE染色观察主动脉病理学改变及内膜增生水平,Western blot检测腹主动脉中p-PI3K和p-Akt的蛋白表达水平。结果:M组中总胆固醇(TC)和低密度脂蛋白(LDL)度均显著高于CL组(均P<0.05);NO和e NOS显著降低(均P<0.05),病理学显示内膜增厚,斑块面积增加。Western blot显示腹主动脉组织中p-PI3K、p-Akt的蛋白表达水平均低于CL组(均P<0.05)。DL组、RS组和IT组与M组相比均降低TC、LDL,升高NO、e NOS水平,上调PI3K、p-Akt的蛋白表达(均P<0.05),IT组的效果最佳(均P<0.05)。结论:丹蒌片联合瑞舒伐他汀具有强化降低兔血脂、改善内皮细胞功能,抗动脉粥样硬化的作用,其作用机制可能与激活PI3/Akt信号通路相关。Objective： To investigate the effect of Danlou Pills on preventing rabbit atherosclerosis plaque from catheter-induced abdominal aortic wall injury with oil ahyperlipidaemic diet and its relationship with PI3 K / Akt signal pathways. Methods：Atherosclerosis was induced in rabbits by maintaining the oil ahyperlipidaemic diet after catheter-induced abdominal aortic wall injury was performed. Forty Japanese male white rabbits were randomly divided into 5 groups with 8 rabbits in each： Control group（ CL）,Model group（ M）,Danlou group（ DL）,Rosuvastatin group（ RS） and intensive group（ IT）. Control group were fed with normal diet and atherosclerosis rabbits were fed with l% cholesterol and 0. 02% Methionine high lipid diet for 9 weeks after catheter-induced abdominal aortic wall injury was performed. NO and e NOS were measured by ELISA kit. The histological changes were observed by microscope with HE staining. Western blot was used to observe p-PI3 K and p-Akt expressions in rabbit model of atherosclerosis. Results： The levels of TC and LDL were increased. NO and e NOS were decreased in M groups. The improvements of TC,LDL,NO and e NOS all were in CL groups,DL groups and IT groups. The p-PI3 K and p-Akt were also increased in DL groups and IT groups compared with those of the model groups（ all P〈0. 05）. IT groups were superior to DL groups and RS groups. Conclusion： The prevention effect of Danlou Pills and Rosuvastation on atherosclerosis may be through PI3 K / Akt signal pathways in a rabbit atherosclerosis model.

关 键 词：动脉粥样硬化 PI3K/AKT 丹蒌片 内皮细胞 

分 类 号：R541.4[医药卫生—心血管疾病]