椎动脉型颈椎病大鼠模型的建立与评价  

Establishment and Evaluation of Rat Model of Cervical Spondylosis of Vertebral Artery Type

作　　者：李清林[1] 宋敏[1] 孙定平 柴居堂 巩彦龙 刘涛[1] 

机构地区：[1]甘肃中医药大学,甘肃兰州730000 [2]甘肃中医药大学第二附属医院,甘肃兰州730000

出　　处：《辽宁中医杂志》2016年第4期861-864,共4页Liaoning Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(813690554);甘肃省重点中医药科研立项课题(GZK-2021-Z14);甘肃省中药现代制药工程研究院项目开发(2305083306);甘肃省高等学校研究生导师科研项目(1206-01)

摘　　要：目的:通过对大鼠行为学、体质量及饮水量的测定,对CSA大鼠混合造模模型进行评价。方法:将90只大鼠随机分为混合造模组、空白对照组及假手术组各30只,通过Morris水迷宫、旷场实验、体质量及饮水量的测定,验证模型是否成功。结果:混合造模组在Morris水迷宫测评中,潜伏期较空白对照组及假手术组均增加(P<0.05);在旷场实验中,混合造模组在站立次数、跨格数、修饰次数、运动总距离、沿边运动距离及中央运动距离等方面分别与空白对照组及假手术组对比,均显著减少(P<0.05);混合造模组较空白对照组及假手术组饮水量减少,体质量增长延迟(P<0.05,P<0.05)。结论:该研究运用混合造模的方法,成功复制出CSA大鼠模型,但仍需影像学及分子生物学等加以佐证。Objective： To evaluate the mixed model of CSA rats by the determination of behavior,body mass and drinking water. Methods： Ninety Wistar rats were randomly divided into mixed modeling group,blank control group and sham operation group,30 rats in each group. Determined by Morris water maze,open field test,body quality and drinking amount,the model success was validated. Results： The mixed model＇s latency in the Morris water maze test was increased compared with that of the blank control group and sham operation group（ P〈0. 05）. In the open field test,the mixed modeling group＇ s standing numbers,number of squares crossed,the modification times,total movement distance and border movement distance and central motor distance were respectively significantly reduced compared with those of the blank control group and sham operation group（ P〈0. 05）. The mixed modeling group＇s amount of water decrease and body weight growth delay were significant compared with those of the blank control group and sham group（ P〈0. 05,P〈0. 05）. Conclusion： This study used the mixed modeling method to replicate CSA rat model successfully,but it still needs to be confirmed by imaging and molecular biology.

关 键 词：混合造模法 椎动脉型颈椎病 WISTAR大鼠 评价 

分 类 号：R681.55[医药卫生—骨科学]