蚕丝的结构与体外降解性质  

In vitro degradation property and structure of silk

作　　者：周婵[1] 曾秀[1] 游华建 王介平[1] 吕金凤[1] 刘彬[2] 黄勇[1] 

机构地区：[1]重庆市畜牧科学院,荣昌402460 [2]西南大学生命科学学院,重庆北碚400715

出　　处：《当代畜牧》2016年第3期49-52,共4页Contemporary Animal Husbandry

基　　金：重庆市农发资金资助项目"功能性蚕丝人工神经体内降解关键技术研究";重庆市基本科研业务费资助项目"丝胶蛋白的改性及血液相容性研究"(2015cstc-jbky-00110);国家自然科学基金资助项目"phrin B2/蚕丝组织工程神经的研究"(31271036)

摘　　要：研究蚕丝的结构与降解性质,不但有利于蚕丝制品的保护,拓展其在生物医药领域的利用空间,还可以为文物的考古提供参考依据。为了探讨蚕丝的结构与降解性质,笔者用扫描电子显微镜和组织切片方法观察蚕茧、茧丝、生丝和熟丝的结构,研究生丝和熟丝分别在4种降解液里156 d中的降解性质。结果显示,生丝中丝胶含量为16.70%~25.00%。经156 d的体外降解,在去离子水、磷酸盐缓冲液（PBS）、细胞培养基（DMEM）、含10%胎牛血清的DMEM中,生丝和熟丝的失重率依次分别为14%/11%、12.5%/12.9%、11.1%/14.3%、8.8%/11.6%。结果表明,蚕丝在水环境中能够被缓慢地降解,降解的实质是水解作用,酶的作用并不显著。避免与化学物质和空气的接触将更有利于丝织品文物的保护。本研究还展示了由丝蛋白到蚕茧和生丝的组装过程。The structure and degradation property of silk have correlations with products of a culture protections and expanding its usage in medicine. To investigate the structure of silk, it was observed with scanning electron microscopy and sections. Silk was immersed in four types of degradation solutions for 156 d to observe its degradation properties. The weight loss ratio of raw silk and degummed raw silk in water, PBS, DMEM and DMEM containing 10% FBS（F-DMEM） were, respectively, 14%/11%, 12.5%/12.9%, 11.1%/14.3%, 8.8%/ 11.6%. These findings suggest that, in the process of degradation of silk, the role of the enzyme is not significant. This study also shows the developing process of cocoons and silk.

关 键 词：蚕丝 文物 结构 降解 酶 

分 类 号：S886.7[农业科学—特种经济动物饲养] TS143.2[农业科学—畜牧兽医]