miR-148b-3p对施万细胞增殖的影响  

The role of miR-148b-3p in Schwann cell proliferation

作　　者：钱天梅[1] 王靖[1] 周松林[1] 姚淳[1] 

机构地区：[1]南通大学江苏省神经再生重点实验室神经再生协同创新中心,南通226001

出　　处：《南通大学学报（医学版）》2015年第6期498-501,共4页Journal of Nantong University(Medical sciences)

基　　金：国家自然科学基金青年基金资助项目(31500823);南通大学校级自然科学类科研基金一般项目(13040397)

摘　　要：目的 :探讨microRNA-148b-3p(miR-148b-3p)对施万(Schwann)细胞增殖的影响及作用的靶基因。方法 :采用Edu染色法检测miR-148b-3p对原代培养的施万细胞增殖能力的影响;基因芯片及生物信息学分析筛选miR-148b-3p可能作用的靶基因;实时定量PCR检测施万细胞中过表达miR-148b-3p模拟物后靶基因的表达变化。结果 :miR-148b-3p能明显促进原代培养的施万细胞的增殖,miR-148b-3p过表达可抑制其靶基因Alcam、Cpd、Dedd、Nptx2、Phf20、Ptpn14和Stard 13的表达。结论 :miR-148b-3p有可能通过对增殖相关靶基因的负调控来影响施万细胞的增殖能力,从而促进周围神经损伤修复。Objective： To investigate the target genes effect of microRNA-148b-3p（miR-148b-3p） in Schwann cell proliferation.Methods： Edu staining analyses were performed to examine the proliferation of primary cultured Schwann cells after transfecting miR-148b-3p mimics. Microarray analysis and bioinformatic prediction were used to choose potential target genes of miR-148b-3p. And the expressions of target genes after miR-148b-3p mimics transfection on Schwann cells were detected by quantitative real-time PCR. Results： miR-148b-3p could significantly promote the proliferation of primary cultured Schwann cells by suppressing the expression of its target genes（Alcam,Cpd,Dedd, Nptx2, Phf20, Ptpn14 and Stard13）.Conclusion： miR-148b-3p could affect Schwann cell proliferation by negatively regulating its target genes involving in proliferation, and facilitate peripheral nerve regeneration.

关 键 词：microRNA-148b-3p 施万细胞 增殖 周围神经损伤修复 

分 类 号：R329.28[医药卫生—人体解剖和组织胚胎学]