抑制CCoAOMT表达对烟草木质素生物合成的影响  

Effects of Repression of CCoAOMT Expression on Lignin Biosynthesis in Tobacco

作　　者：刘惠荣[1] 赵华燕[2] 魏建华[3] 邵金旺[1] 朱至清 宋艳茹 

机构地区：[1]内蒙古农业大学,呼和浩特010018 [2]光合作用和环境分子生理学重点实验室,中国科学院植物研究所,北京100093 [3]北京农业生物技术研究中心,北京100089

出　　处：《中国农业科学》2002年第8期921-924,共4页Scientia Agricultura Sinica

基　　金：国家自然科学基金项目 ( 30 170 783) ;国家"86 3"资助项目 ( 2 0 0 1AA2 12 15 1)

摘　　要：通过农杆菌介导法将毛白杨CCoAOMTcDNA反向导入烟草。PCR、PCR Southern和Northern点杂交检测表明 ,CCoAOMTcDNA已反向整合到烟草基因组中并在转录水平表达。将转基因株系移栽温室 3个月后 ,以Klason法测下部茎木质素含量。与对照相比 ,大部分转基因株系木质素含量均有不同程度的下降 ,其中下降最多达 37%,表明抑制植物内源CCoAOMT的表达可有效地降低木质素含量。Antisense CCoAOMT cDNA of Chinese white poplar(Populus tomentosa) was transformed into tobacco W38 mediated by Agrobacterium tumefaciens. The results of PCR, PCR-Southern and Northern dot analysis indicated that the antisense CCoAOMT cDNA had integrated in the transgenic tobacco genome and expressed at transcriptional level. The basic stems of the 3-month-old transgenic plants growing in the green house were cut off and dried for Klason lignin analysis. Lignin content of most of the transgenic plants declined compared with the control plants, and one of them, the lignin content decreased by 37%. Our results suggest that the suppression of endogenous CCoAOMT expression can reduce lignin content of the plants effectively.

关 键 词：抑制 CCOAOMT 表达 烟草 木质素 生物合成 毛白杨 环保型制浆树种 

分 类 号：Q943.2[生物学—植物学]