Standardization of experimental animals temporal bone sections  

作　　者：Dalian Ding Jintao Yu Peng Li Kelei Gao Haiyan Jiang Wenjuan Zhang Hong Sun Shankai Yin Richard Salvi 

机构地区：[1]Center for Hearing and Deafness, Department of Communicative Disorders and Sciences, University of New York at Buffalo [2]Department of Otolaryngology Head and Neck Surgery, Sun Ya-sen University Third Hospital [3]Department of Otolaryngology Head and Neck Surgery, the Sixth People's Hospital of Shanghai Jiao Tong University [4]Department of Otolaryngology Head and Neck Surgery, Central South University Xiangya Hospital [5]Department of Otolaryngology Head and Neck Surgery, Union Hospital, Tongji Medical College of Huazhong University of Science & Technology

出　　处：《Journal of Otology》2015年第2期66-71,共6页中华耳科学杂志（英文版）

摘　　要：Preparation of the temporal bone for light microscopy is an important step in histological studies of the inner ear. Due to the complexity of structures of the inner ear, it is difficult to measure or compare structures of interest without a commonly accepted standardized measure of temporal bone sections. Therefore, standardization of temporal bone sections is very important for histological assessment of sensory hair cells and peripheral ganglion neurons in the cochlear and vestibular systems. The standardized temporal bone sectioning is oriented to a plane parallel to the outer and internal auditory canals. Sections are collected from the epitympanum to the hypotympanum to reveal layers in the order of the crista ampullaris of the superior and lateral semicircular canals, macula utriculi and macula sacculi, superior vestibular ganglion neurons, macula of saccule and inferior vestibular ganglion neurons, cochlear modiolus, endolymphatic duct and endolymphatic sac, and finally the crista ampullaris of the posterior semicircular canal. Moreover, technical details of preparing for temporal bone sectioning including fixation, decalcification, whole temporal bone staining, embedding penetration, and embedding orientation are also discussed.Preparation of the temporal bone for light microscopy is an important step in histological studies of the inner ear. Due to the complexity of structures of the inner ear, it is difficult to measure or compare structures of interest without a commonly accepted standardized measure of temporal bone sections. Therefore, standardization of temporal bone sections is very important for histological assessment of sensory hair cells and peripheral ganglion neurons in the cochlear and vestibular systems. The standardized temporal bone sectioning is oriented to a plane parallel to the outer and internal auditory canals. Sections are collected from the epitympanum to the hypotympanum to reveal layers in the order of the crista ampullaris of the superior and lateral semicircular canals, macula utriculi and macula sacculi, superior vestibular ganglion neurons, macula of saccule and inferior vestibular ganglion neurons, cochlear modiolus, endolymphatic duct and endolymphatic sac, and finally the crista ampullaris of the posterior semicircular canal. Moreover, technical details of preparing for temporal bone sectioning including fixation, decalcification, whole temporal bone staining, embedding penetration, and embedding orientation are also discussed.

关 键 词：Experimental animal Temporal bone section Temporal bone Collodion embedding DECALCIFICATION 

分 类 号：R764[医药卫生—耳鼻咽喉科]