高效液相色谱法同时测定森登-4有效部位中7种成分的含量  

Simultaneous determination of the content of 7 effeetive components in Sendeng-4

作　　者：白埔 萨础拉[1] 董玉[1] 

机构地区：[1]内蒙古医科大学,呼和浩特010059

出　　处：《药物分析杂志》2016年第4期602-606,共5页Chinese Journal of Pharmaceutical Analysis

基　　金：国家自然科学基金(No.81160550);内蒙古自然科学基金(No.2013JQ03)

摘　　要：目的：建立HPLC法同时测定森登-4有效部位中7种成分的含量。方法：采用Extend-C18（250mm×4.6 mm,5μm）色谱柱,以乙腈（A）-0.6%磷酸水溶液（B）为流动相,梯度洗脱,流速0.5 m L·min-1,柱温27℃,检测波长为254 nm和292 nm,进样量10μL。结果：没食子酸、京尼平-1-β-D-龙胆二糖苷、栀子苷、（2R,3R）-双氢杨梅素、芦丁、杨梅素、槲皮素的质量浓度与其峰面积具有良好的线性关系（r=0.999 9）;平均回收率为97.30%～103.9%,RSD为0.08%～1.00%。没食子酸、京尼平-1-β-D-龙胆二糖苷、栀子苷、（2R,3R）-双氢杨梅素、芦丁、杨梅素、槲皮素平均含量分别为0.379%、0.447%、2.740%、3.965%、0.253%、0.878%、0.044%。结论：经方法学验证,本法可用于森登-4有效部位的化学成分含量测定。Objective：To establish an HPLC method for simultaneous determination of the content of 7 effective components in Mongolian medicine Sendeng-4.Methods：The Extend-C18 column（250 mm×4.6 mm,5 μm）was adopted with a mobile phase consisting of acetonitrile（A）and 0.6% phosphoric acid aqueous solution（B）with gradient elution at the flow rate of 0.5 m L· min-1.The column temperature was 27 ℃,the detection wavelengths were 254 nm and 292 nm,and the injection volume was 10 μL.Results：Mass concentration and the peak area of gallic acid,genipin-1-β-D-gentiobioside,geniposide,（2R,3R）-dihydromyricetin,rutin,myricetin and quercetin exhibited a good linear relationship with correlation coefficient of 0.999 9,the average recovery was 97.30%-103.94% and the RSD was 0.08%-1.00%.The average contents of gallic acid,genipin-1-β-Dgentiobioside,geniposide,（2R,3R）-dihydromyricetin,rutin,myricetin,quercetin of 6 parts test solution were 0.379%,0.447%,2.740%,3.965%,0.253%,0.878%,and 0.044%,respectively.Conclusion：The results of methodology validation showed that the established method can be applied to the determination of chemical constituent of Mongolian medicine Sendeng-4.

关 键 词：高效液相色谱法 蒙药 森登-4 有效部位 没食子酸 京尼平-1-β-D-龙胆二糖苷 栀子苷 (2R 3R)-双氢杨梅素 芦丁 杨梅素 槲皮素 

分 类 号：R917[医药卫生—药物分析学]