差示分光光度法测定独一味胶囊中总黄酮含量  

Determination of total flavonoids in Kudo capsule by differential spectrophotometry

作　　者：郭爱玲[1] 张淑蓉[1] 刘晓芳[1] 李虹[1] 焦建华[1] 裴晓丽[1] 

机构地区：[1]山西中医学院,山西太原030024

出　　处：《山西中医学院学报》2015年第6期16-17,共2页Journal of Shanxi College of Traditional Chinese Medicine

基　　金：山西中医学院"十一五"科技产业规划项目(2006);山西省科技攻关项目(20120313015-4)

摘　　要：目的:测定藏药独一味胶囊中总黄酮含量。方法:以芦丁为观测指标,在517 nm采用差示分光光度法直接测定,不经分离无干扰。结果:对照品在9.78μg/mL^68.46μg/mL的浓度范围内,差示吸收值(_△A)与浓度呈良好线性关系,r=0.999 7,回归方程为_△A=9.637 5C+0.001 7,平均加样回收率为100.31%,RSD=2.13%(n=6)。结论:该测定方法便于操作,结果准确,重现性良好,可用于藏药独一味胶囊的质量控制。Objective：To determine total flavonoids in Tibetan Kudo capsule. Methods：Differential spectrophotometry was applied with lutin as standard index at the wavelength of 517 nm without prior separation. Results：The liner range of absorbance and the control concentration was 9.78 μg/mL ~68.46 μg/mL,r =0.999 7. The average recovery was 100.31% and RSD was 2.13%（n=6）. Conclusion：The method is simple,accurate,reproducible and suitable for the quality control of Tibetan Kudo capsule.

关 键 词：独一味胶囊 总黄酮 差示分光光度法 

分 类 号：R285[医药卫生—中药学]