参附汤对心脏毒性损伤大鼠细胞凋亡相关基因的影响  

Effects of Shenfu on Apoptosis Related Genes in Rats with Cardial Injury

作　　者：庄华梅[1] 陈秀娇[2] 林灿[2] 王俊芳[1] 万杰[1] 蔡学明[2] 

机构地区：[1]莆田学院药学与医学技术学院,福建莆田351100 [2]莆田学院基础医学部,福建莆田351100

出　　处：《莆田学院学报》2016年第2期10-14,共5页Journal of putian University

基　　金：福建省教育厅资助项目(JA13288);莆田市科技项目(2014S02(2));莆田学院科研基金项目(2013010);莆田学院育苗基金项目(2013053);莆田学院大学生创新创业项目(201511498054)

摘　　要：探讨参附汤对阿霉素心脏毒性损伤大鼠细胞凋亡相关基因转录水平的影响。采用实时荧光定量PCR法测定大鼠心肌组织Bax、Bcl-2和Caspase-3 m RNA的转录水平。结果表明,阿霉素心脏毒性损伤模型大鼠与对照组相比,Bax和Caspase-3 m RNA的转录水平均提高,而Bcl-2 m RNA的转录水平降低,差异有统计学意义(P<0.05)。参附汤组与模型组相比,Bax和Caspase-3 m RNA的转录水平均降低,而Bcl-2 m RNA的转录水平提高,差异有统计学意义(P<0.05)。阿霉素心脏毒性损伤与心肌细胞凋亡有关,参附汤通过调节细胞凋亡相关基因表达水平来改善心肌毒性损伤,达到保护心肌的功能。The paper investigated the effect of Shenfu on cardiomyocyte apoptosis of cardial injury induced by adriamycin in rats. Real-time quantitative PCR（QPCR） was applied to measure the transcriptions of Bax, Bcl-2 and Caspase-3 in myocardial tissue. Compared with control group, in adryamycin-induced rat group the transcriptions of Bax and Caspase-3 increased and the transcription of Bcl-2 decreased, with statistical significance（P〈0.05）. Compared with adryamycin-induced group, in Shenfu group the transcriptions of Bax and Caspase-3 decreased and the transcription of Bcl-2 increased, with statistical significance（P〈0.05）. Cardial injury induced by adriamycin in rat was associated with myocardial cell apoptosis. Shenfu can protect myocardium by regulating cell apoptosis related genes expressions.

关 键 词：参附汤 阿霉素 心脏毒性损伤 细胞凋亡 基因表达 实时荧光定量PCR 

分 类 号：R285.5[医药卫生—中药学]