荧光原位杂交技术在套细胞淋巴瘤石蜡切片的应用研究  

Applied research of fluorescence in situ hybridization in paraffin section of mantle cell lymphoma

作　　者：吴蔚[1] 倪军[1] 王红[1] 孙幸[1] 方悦之[1] 顾健[1] 

机构地区：[1]江苏省苏北人民医院,扬州市血液学研究所实验室,江苏扬州225001

出　　处：《实验与检验医学》2016年第2期134-136,共3页Experimental and Laboratory Medicine

基　　金：国家自然科学基金项目(编号:81170104)

摘　　要：目的建立淋巴瘤石蜡切片应用荧光原位杂交(fluorescence in situ hybridization,FISH)检测的操作流程,探讨其在套细胞淋巴瘤(mantle cell lymphoma,MCL)诊断中的应用价值。方法优化石蜡切片FISH检测步骤和实验条件,在16例病理拟诊断MCL的石蜡切片上应用FISH检测IGH/CCND1融合基因。结果确立了本实验室石蜡切片FISH检测的实验方法和条件;拟诊断MCL的病例中IGH/CCND1融合基因阳性检出率为75%(12/16)。结论淋巴瘤石蜡切片FISH检测可满足临床应用要求;应用FISH技术检测IGH/CCND1融合基因有助于MCL的诊断。Objective To establish the operation process of fluorescence in situ hybridization（FISH） in paraffin section of lymphoma,and explore the application of FISH in diagnosis of mantle cell lymphoma（MCL）. Methods The testing steps and experimental conditions of FISH in paraffin section were optimized,and 16 cases of quasi diagnosis of MCL were detected IGH/CCND1 fusion gene by FISH in paraffin section. Results The experiment methods and conditions of FISH in paraffin section were successfully established in our laboratory,and the positive detection rate of IGH/CCND1 fusion gene was 75%（12/16） in quasi diagnosis cases of MCL. Conclusion It can meet the requirements of clinical application by FISH in paraffin section of lymphoma;and the detection of IGH/CCND1 fusion gene by FISH is useful for the diagnosis of MCL.

关 键 词：荧光原位杂交 石蜡包埋 套细胞淋巴瘤 IGH/CCND1 

分 类 号：R446.8[医药卫生—诊断学] R551.2[医药卫生—临床医学]