呫吨酮衍生物XP-15对BEL-7402细胞线粒体膜电位的影响  

Effect of xanthono-pyridine derivative XP-15 on Human Gastric Carcinoma BEL-7402 cells

作　　者：陈毅飞[1] 胡龙美 张楠[2] 梁祖鹏 戴支凯[1] 

机构地区：[1]桂林医学院药理学教研室,广西桂林541004 [2]桂林医学院药学院,广西桂林541004

出　　处：《科技视界》2016年第11期84-85,共2页Science & Technology Vision

基　　金：广西自然科学基金(2013GXNSFBA019176);广西高校科研资助项目一般项目(2013YB157)

摘　　要：目的 :探讨呫吨酮并吡啶衍生物9-(2-吗啡啉乙酰胺基)-7H-吡啶并[4,3-c]呫吨-7-酮(XP-15)对BEL-7402细胞线粒体膜电位的影响。方法:通过MTT法、细胞形态学和克隆实验观察XP-15对BEL-7402细胞增殖的影响;用荧光分光光度计检测线粒体膜电位的影响。结果:高剂量的XP-15能明显抑制BEL-7402细胞增殖(P<0.05);对BEL-7402细胞克隆的抑制作用,呈剂量和时间依赖性。XP-15作用后,BEL-7402细胞的线粒体膜电位降低(P<0.05)。结论:XP-15诱导BEL-7402细胞凋亡的作用,可能与其降低线粒体膜电位有关。Objective： To investigate mitochondria membrane potential effect of a new xanthono-pyridine derivative 2-morpholino-N-（7-oxo-7H-chromeno[3,2-h]quinolin-9-yl）acetamide（XP-15）on Human Gastric Carcinoma cell line BEL-7402. Methods： Antiproliferative effect of XP-15 on BEL-7402 cells was evaluated by MTT assay, morphological examination and colonial assay. Intracellular mitochondria membrane potential were detected by fluorospectrophotometer. Results： XP-15 could inhibit proliferation of BEL-7402 cells in dose- and time-dependent manner（P0.05）. After treated with XP-15, mitochondria membrane potential（P0.05）of BEL-7402 cells were decreased. Conclusion： XP-15 inducing BEL-7402 cells apoptosis might be associated with decreasing mitochondria membrane potential.

关 键 词：9-(2-吗啡啉乙酰胺基)-7H-吡啶并[4 3-c]呫吨-7-酮 人肝癌BEL-7402细胞 线粒体膜电位 

分 类 号：Q51[生物学—生物化学]