接种丛枝菌根真菌对小麦根内氮转运蛋白基因表达的影响  

Effect of inoculating different arbuscular mycorrhizal fungi on the expression of nitrogen transporter genes in roots of wheat

作　　者：段建锋[1] 田慧[1] 高亚军[1] 

机构地区：[1]农业部西北植物营养与农业环境重点实验室/西北农林科技大学资源环境学院,陕西杨凌712100

出　　处：《中国土壤与肥料》2016年第2期130-136,共7页Soil and Fertilizer Sciences in China

基　　金：国家自然科学基金项目(41201239);陕西省人才专项配套经费(201104050280);西北农林科技大学博士启动金项目(201104050279);西北农林科技大学"创新团队建设计划";国家小麦现代产业技术体系建设专项经费;教育部"新世纪优秀人才支持计划"项目(NCET-08-0465)

摘　　要：通过盆栽试验,研究了在低氮(不施氮)和高氮(施氮0.2 g·kg^(-1))水平下接种不同种类丛枝菌根(AM)真菌[Funneliformis mosseae(BGC-NM03D)、Claroideoglomus etunicatum(BGC-NM01B)和Rhizophagus intraradices(BJ09)]对小麦生长、氮吸收及根内4个硝态氮转运蛋白(NRT)基因、1个辅助蛋白(NAR)基因和2个铵态氮转运蛋白(AMT)基因表达的影响。结果表明,3种AM真菌均能够侵染小麦根系,以R.intraradices菌根的侵染率最高;接种R.intraradices或C.etunicatum能够显著提高小麦的生物量或地上部氮吸收量;无论是高氮还是低氮处理,接种AM真菌后均显著下调了小麦根内NRT、NAR和AMT基因的表达水平,且不同AM真菌调控小麦根内氮转运蛋白基因表达的能力具有明显差异。A pot experiment was carried out to investigate the effects of inoculation of different arbuscular mycorrhizal（ AM）fungi [Funneliformis mosseae（ BGC-NM03D）, Claroideoglomus etunicatum（ BGC-NM01B）, Rhizophagus intraradices（ BJ09） ] on growth,nitrogen uptake and expression of 5 nitrate transporter（ NRT NAR） and 2 ammonium transporter（ AMT） genes in roots of wheat under low N（ no N application） and high N（ N 0. 2 g·kg^-1soil） treatments. The results indicated that wheat roots were colonized by all the three AM fungi,and the fungus R. intraradices had the highest colonization rate in both N treatments. The inoculation of R. intraradices and C. etunicatum significantly increased shoot biomass or shoot N accumulation compared with the non-mycorrhizal control. The transcription levels of all the NRT and AMT genes in roots of wheat were down-regulated by inoculating the AM fungi in both N treatments,and different AM fungi exhibited different abilities to regulated the expression of the NRT,NAR and AMT genes.

关 键 词：小麦 丛枝菌根真菌 氮 基因表达 转运蛋白基因 

分 类 号：S512.1[农业科学—作物学]