PM_(2.5)对运动大鼠肺氧化损伤和炎症反应相关指标的影响  

Effects of PM_(2.5) on factors related to oxidative damage and inflammation in lungs of exercised rats

作　　者：李瑞金[1] 赵利芳[1] 王振涛[1] 翟苗苗[1] 白云龙[1] 张全喜[1] 李君灵[2] 

机构地区：[1]山西大学环境科学研究所,山西太原030006 [2]山西财经大学体育学院

出　　处：《环境与健康杂志》2016年第2期100-102,共3页Journal of Environment and Health

基　　金：山西省自然科学基金(2014011036-2);山西省高等学校科技创新项目(20121008;2014110);山西省回国留学人员科研项目(2013-016)

摘　　要：目的探讨细颗粒物(PM_(2.5))对运动大鼠肺氧化损伤和炎症相关指标的影响。方法将20只健康清洁级雄性Wistar大鼠随机分为4组,分别为生理盐水对照组、PM_(2.5)组、PM_(2.5)+运动组、PM_(2.5)+运动加强组,每组5只。PM_(2.5)组气道滴注染毒剂量为6.0 mg/kg,每周染毒5 d,每天1次,休息2 d;连续4周;PM_(2.5)+运动组在滴注PM_(2.5)后游泳1 h;PM_(2.5)+运动加强组大鼠先游泳4周,再滴注染毒和1 h游泳。测定肺组织中氧化损伤指标[肺总抗氧化能力(T-AOC)、血红素氧合酶-1(HO-1)、一氧化氮(NO)]和炎症相关指标[肿瘤坏死因子-α(TNF-α)、表面活性物质蛋白(SP-A)]的水平。结果与对照组相比,PM_(2.5)组、PM_(2.5)+运动组和PM_(2.5)+运动加强组大鼠肺组织中T-AOC和SP-A的含量均下降,而HO-1的活力及NO、TNF-α的含量均较高,差异有统计学意义(P<0.05,P<0.01);而PM_(2.5)+运动加强组大鼠肺组织中NO的含量无明显变化。与PM_(2.5)组相比,PM_(2.5)+运动组大鼠肺组织中T-AOC降低,而HO-1活力和NO的含量较高,差异均有统计学意义(P<0.05);而PM_(2.5)+运动加强组大鼠肺组织中的T-AOC和HO-1的活力及NO的含量均无明显改变。与PM_(2.5)+运动组相比,PM_(2.5)+运动加强组大鼠肺T-AOC水平有所回升,而HO-1活力和NO的含量均降低,差异有统计学意义(P<0.05,P<0.01)。与PM_(2.5)组比较,PM_(2.5)+运动组大鼠肺组织中TNF-α的含量均增加,差异有统计学意义(P<0.05)。PM_(2.5)组、PM_(2.5)+运动组和PM_(2.5)+运动加强组大鼠肺组织中SP-A的含量间比较,差异无统计学意义(P>0.05)。结论 PM_(2.5)可诱发大鼠肺组织氧化损伤和炎症效应,PM_(2.5)暴露下运动会加剧此效应。适宜的加强运动在一定程度上减轻了PM_(2.5)污染造成的肺组织损伤。Objective To understand the effects of fine particulate matter（PM2.5） on the factors related to oxidative damage and inflammation of lungs of exercised rats. Methods Male Wistar rats were randomly divided into four groups including quiet control,PM2.5group,PM2.5＋exercise group and PM2.5＋exercise enhancement group, five rats in each group. The PM2.5suspensions（6.0 mg/kg body weight） were given to rats by the intratracheal instillation, once a day for five days,and then rest two days,repeating this for four weeks. Meanwhile,the rats in PM2.5＋exercise group swam 1 h in pools after PM2.5suspension intratracheal instillation. The rats of PM2.5＋exercise enhancement group were pre-trained for four weeks in swimming pools,and then PM2.5exposure plus swimming were implemented like PM2.5＋exercise group. The levels of oxidative damage indexes（T-AOC,HO-1,NO） and inflammation indexes（TNF-α,SP-A） in the lungs were measured. Results The levels of T-AOC and SP-A decreased significantly,while the levels of TNF-α,HO-1 and NO increased markedly in three treatment groups compared with the control. Such effects in PM2.5＋exercise group were significant compared to that in PM2.5group. However,the antioxidant enzymes had a rising trend and the TNF-α and NO contents were decreased in PM2.5＋exercise enhancement group compared with PM2.5＋exercise group. Conclusion PM2.5may induce lung oxidative damage and inflammation,and exercise combined with PM2.5exposure may aggravate such effects. To some extent,enhancing the athletic ability may mitigate the lung injury caused by PM2.5pollution.

关 键 词：PM2.5 细颗粒物 运动大鼠 肺 氧化损伤 炎症 

分 类 号：R994.6[医药卫生—毒理学]