粉尘接触人群血清微小RNA表达研究  

MicroRNA expression in the serum of dust exposure population

作　　者：张明[1] 曾强[1] 杨德一[1] 刘义涛[1] 王欣[1] 刘保峰[1] 李培[1] 夏俊娣[2] 张露新 谢连宏 

机构地区：[1]天津市疾病预防控制中心,天津300011 [2]天津市职工医院,300182 [3]天津市东丽区疾病预防控制中心,300300 [4]天津市西青区疾病预防控制中心,300112

出　　处：《中国职业医学》2016年第2期178-180,共3页China Occupational Medicine

基　　金：天津市科技支撑重点项目(11ZCGYSY02100);天津市自然科学基金(13JCZDJC30600;15JCQNJC45100);天津市卫生局科技基金重点项目(2014KR13)

摘　　要：目的分析粉尘接触人群血清微小RNA(miRNAs)水平。方法采用整群随机抽样方法,选择479名粉尘接触作业工人作为接尘组,434名无职业性粉尘接触史的健康人员作为对照组,43名尘肺病患者作为尘肺组。采集3组人群周围静脉血分离血清,采用实时定量聚合酶链反应法检测miR-16、miR-21、miR-29a、miR-155、miR-200c、miR-204和miR-206的相对表达水平,观察3组人群miRNAs表达的差异。结果 3组人群miR-16、miR-29a和miR-200c相对表达水平从高至低依次为尘肺组<接尘组<对照组(P<0.05),miR-204相对表达水平从高至低依次为对照组<接尘组<尘肺组(P<0.05)。接尘组和尘肺组人群miR-21相对表达水平均高于对照组(P<0.05),接尘组人群miR-155相对表达水平低于对照组(P<0.05),尘肺组人群miR-206相对表达水平分别高于对照组和接尘组(P<0.05)。结论 miR-16、miR-29a、miR-200c和miR-204可作为尘肺病早期诊断的生物标志物。Objective To analyze the microRNA levels in the serum of dust exposure population. Methods By cluster random sampling method,479 dust exposure workers were selected as dust exposure group. Four hundred and thirty-four normal healthy people without occupational dust exposure history were selected as control group. Forty-three pneumoconiosis patients were selected as pneumoconiosis group. The peripheral venous blood of the objects of 3 groups were collected,and the plasma serum were separated for detecting the relative expression of miR-16,miR-21,miR-29 a,miR-155,miR-200 c,miR-204 and miR-206 in serum by real time quantitative polymerase chain reaction. The difference of microRNA expression in the above 3 groups was observed. Results From high to low,the relative expression levels of miR-16,miR-29 a and miR-200 c were pneumoconiosis group dust exposure group control group（ P〈0. 05）,while the relative expression levels of miR-204 were control group dust exposure group pneumoconiosis group（ P〈0. 05）.The relative expression levels of miR-21 in dust exposure and pneumoconiosis groups were higher than that of control group,respectively（ P〈0. 05）. The relative expression level of miR-155 in dust exposure group was lower than that of control group（ P〈0. 05）. The relative expression level of miR-206 in pneumoconiosis group was higher than those of dust exposure group and control group respectively（ P〈0. 05）. Conclusion The miR-16,miR-29 a,miR-200 c and miR-204 could be used as biomarkers in early diagnosis of pneumoconiosis disease.

关 键 词：微小RNA 粉尘 尘肺病 血清 生物标志物 

分 类 号：R135.2[医药卫生—劳动卫生]