溶剂解吸-气相色谱法同时测定工作场所空气中环己烷与甲基环己烷  

Simultaneous determination of cyclohexane and methylcyclohexane in workplace air by solvent desorption-gas chromatography

作　　者：李行方[1] 尹江伟[1] 付斌[1] 钟伟燕[1] 吴礼康[1] 

机构地区：[1]深圳市宝安区疾病预防控制中心,广东深圳518101

出　　处：《中国职业医学》2016年第2期201-204,共4页China Occupational Medicine

摘　　要：目的改进工作场所空气中环己烷与甲基环己烷的溶剂解吸-气相色谱测定方法。方法采用活性炭管采集样品,二硫化碳解吸,经DB-1毛细管色谱柱分离,火焰离子化检测器检测,标准曲线法定量。结果本方法环己烷与甲基环己烷的质量浓度分别在1.0～1 402.2与0.8～1 999.4 mg/L范围内呈线性关系,相关系数均为0.999 9;检出限均为0.3 mg/L,定量下限分别为1.0和0.8 mg/L,最低检出浓度均为0.2 mg/m^3,最低定量浓度分别为0.7和0.6 mg/m^3（以采集1.5 L工作场所空气计）;平均解吸效率分别为98.5%～99.3%和97.6%～99.0%;批内相对标准偏差（RSD）分别为0.36%～0.59%与0.34%～0.50%,批间RSD分别为0.89%～2.04%与0.87%～2.22%;样品在室温下可保存7 d。结论本方法灵敏度高,精密度好,操作简单,可用于同时测定工作场所空气中环己烷与甲基环己烷。Objective To explore an improved method for determination of cyclohexane and methylcyclohexane in workplace air by solvent desorption-gas chromatography. Methods Cyclohexane and methylcyclohexane in workplace air were collected by activated carbon tubes,desorbed with carbon disulfide,separated by DB-1 capillary chromatography column,detected by flame ionization detector and quantified using the standard calibration curves. Results The linear range of the concentration of cyclohexane and methylcyclohexane were 1. 0-1 402. 2 and 0. 8-1 999. 4 mg / L respectively.Both the correlation coefficients were 0. 999 9. Both the detection limits were 0. 3 mg / L. The limits of quantification were1. 0 and 0. 8 mg / L respectively. Both the minimum detectable concentrations were 0. 2 mg / m^3. The minimum quantitative mass concentrations of cyclohexane and methylcyclohexane were 0. 7 and 0. 6 mg / m^3respectively（ sample volume was 1. 5L）. The average desorption efficiencies were 98. 5%-99. 3% and 97. 6%-99. 0% respectively. The relative standard deviations（ RSD） of within-run precision were 0. 36%-0. 59% and 0. 34%-0. 50% respectively. The RSD of between-run precision were 0. 89%-2. 04% and 0. 87%-2. 22% respectively. The samples could be stored for up to 7 days at room temperature. Conclusion This method has features of simple operation,high sensitivity and good precision,which is suitable for simultaneous determination of cyclohexane and methylcyclohexane in workplace air.

关 键 词：环己烷 甲基环己烷 工作场所空气 气相色谱法 溶剂解吸 

分 类 号：R134.4[医药卫生—劳动卫生]