杜仲黄酮对小鼠淋巴细胞增殖及IL-2和IFN-γ诱生的影响  

Effects of Flavonoid and Flavone from Eucommia ulmoides Oliv.on Proliferation of Splenocytes and Production of IL-2 and IFN-γ in Mice

作　　者：王志宏[1] 雷明盛[1,2,3] 彭胜[1] 郑阳[1] 史丽娟[1] 彭密军[1] 

机构地区：[1]吉首大学林产化工工程湖南省重点实验室杜仲综合利用国家地方联合工程实验室湖南省植物功能成分利用协同创新中心,张家界427000 [2]武汉大学中南医院,武汉430071 [3]湖南省张家界市人民医院,张家界427000

出　　处：《天然产物研究与开发》2016年第4期514-518,489,共6页Natural Product Research and Development

基　　金：湖南省教育厅高校产业化培育项目(11CY015);吉首大学林产化工工程湖南省重点实验室开放项目(JDZ201501)

摘　　要：采用MTT法和ELISA技术,通过考察不同纯度的杜仲黄酮对小鼠脾淋巴细胞增殖及细胞因子IL-2和IFN-γ诱生作用的影响,初步探讨杜仲黄酮的免疫活性。结果表明,杜仲黄酮粗品及槲皮素均对经Con A或LPS刺激下的小鼠脾淋巴细胞的增殖和细胞因子IL-2与IFN-γ的分泌有协同作用;随细胞培养时间延长,与空白组比较作用效果也逐渐显著。多重显著性分析结果显示,在一定范围内,不同浓度的样品对脾淋巴细胞的增殖效果存在差异,并有一定的浓度依赖性。而山奈酚则随浓度的升高对淋巴细胞的增殖呈抑制趋势,同时对细胞因子的诱生起下调作用。In this study,MTT and ELISA methods were used to investigate the effects of flavonoid and flavone from Eucommia ulmoides Oliv. on prolifeation of splenocytes and production of IL-2 and IFN-γ in mice. The results showed that flavonoid extract of E. ulmoides Oliv. and quercetin can siganificantly upregulate prolifeation and production of IL-2 and IFN-γ in lymphocytes stimulated by Con A or LPS. Along with the extension of time,compared with the blank group,the experimental results changed significantly. The results of multiple significance analysis showed that there was certain dose concentration dependence. However,kaempferol can effectively inhibit proliferation and production of cytokine in a certain concentration range.

关 键 词：杜仲黄酮 槲皮素 山奈酚 免疫 淋巴细胞 

分 类 号：R285.5[医药卫生—中药学] R392.1[医药卫生—中医学]