分光光度法测定血浆中黑色素含量的方法学研究  

Determination of Melanin in Plasma by UV-VIS Spectrophotometry

作　　者：张朋俊[1] 杨润琳[2] 杨敏[1,2,3] 柏志成 李晓天[1] 

机构地区：[1]郑州大学药学院,郑州450000 [2]江苏省原子医学研究所分子影像中心,无锡214063 [3]南京医科大学,南京210000

出　　处：《天然产物研究与开发》2016年第4期525-528,535,共5页Natural Product Research and Development

基　　金：国家自然科学基金(51473071;81472749;81401450);十二五重大新药专项(2012ZX09505-001-001);江苏省科技厅资助项目(BE2014609);江苏省卫生厅资助项目(Q201406)

摘　　要：黑色素纳米材料（MNP）易与金属离子螯合,本文建立了分光光度法测定铁过载模型鼠血浆中MNP含量的方法,为MNP治疗铁过载提供基础。MNP标准溶液在200-800 nm波长范围内扫描呈全波谱吸收,选定490 nm波长为检测波长,且在5-100μg/m L浓度范围内线性关系良好。不同浓度的Fe^3＋对MNP吸收无影响。该方法快速、操作简便且重现性好,可有效用于血浆中MNP的含量测定。Melanin is easy to chelate metal ions. In this study,an UV-Vis spectrophotometry method as firstly established for the determination of melanin nanomaterials（ MNP） content in plasma of iron overload mouse model,thus provided the basis of MNP for the treatment of iron overload. The standard solution of MNP showed a full spectrum absorption in the wavelength range of 200-800 nm. It had no interference peak at the selected wavelength 490 nm. The good linearity was achieved within the concentration of 5-100 g / m L. Different concentrations of Fe^3 ＋ had no effect on the absorption of MNP. The developed method was rapid,simple and reproducible. It can be effectively used for the determination of MNP in plasma samples.

关 键 词：黑色素 血浆 分光光光度法 铁 

分 类 号：R917[医药卫生—药物分析学]