精子因素对体外受精-胚胎移植术中受精率的影响  

Impact of sperms on fertilization rate after in-vitro fertilization and embryo transfer

作　　者：马兰[1] 刘洋[1] 镡颖[1] 陈静思[1] 杨晓玲[1] 

机构地区：[1]昆明医科大学第二附属医院生殖科,650101

出　　处：《实用医学杂志》2016年第8期1271-1274,共4页The Journal of Practical Medicine

基　　金：云南省教育厅科研基金重点项目(编号:2014z075)

摘　　要：目的:探究体外受精-胚胎移植术(IVF-ET)中受精失败的相关因素。方法:选取接受IVF治疗的患者150例,按照受精率分为完全受精失败组(TFF组,n=50)、低受精率组(LFR组,n=50)和正常对照组(NFR组,n=50)。男性患者采集精子后,观察计数,计算精子浓度、向前精子活动率及正常形态率。Western blot检测精子中生死源性血管紧张素I转换酶(g ACE)的表达水平,Logistic回归检测影响受精率的因素。结果:TFF组的受精率、精子浓度、向前精子活动力和精子正常形态率均低于LFR组和NFR组(P<0.05)。Western blot结果显示,TFF组、LFR组和NFR3组患者g ACE的表达量之间差异具有显著性(P<0.05),g ACE在NFR中的表达水平显著高于TFF组和LFR组(P<0.05);Logistic回归分析结果显示受精率、精子浓度、向前精子活动力、精子正常形态率和g ACE在精子中的表达水平均与受精失败具有相关性,是受精失败的独立危险因素。结论:精子浓度、向前精子活动率和精子正常形态率均影响IVF的成败,g ACE在受精率低的人群中低表达,可作为预测受精失败的潜在生物标志物。Objective To explore the related factors of fertilization failure after in-vitro fertilization and embryo transfer.Methods 150 patients were divided into total fertilization failure（TFF） group,low fertilization（LFR） group,and control（NFR） group according to fertilization rate.Semen was collected from the male patients;the number,concentration,shape,and progressive motility of sperms were measured.Level of g ACE was detected by Western blot.Logistic regression was used to explore the factors affecting fertilization rate.Results The fertilization rate and the concentration,progressive motility,and shape of sperms in were lower TFF group than in LFR group and NFR group（P〈0.05）.Western blot proved that level of g ACE group was higher in TFF than in LFR group and NFR group（P〈0.05）.Logistic regression showed that the fertilization rate,the concentration,progressive motility,shape of sperms,and the level of g ACE were all the independent risk factors for fertilization failure.Conclusions The concentration,progressive motility,and shape of sperms have impact on IVF.A lower expression of g ACE in patients with lower fertilization rate can be used as a potential biomarker for predicting fertilization failure.

关 键 词：体外受精-胚胎移植 失败 生殖源性血管紧张素Ⅰ转换酶 

分 类 号：R714.8[医药卫生—妇产科学]