立枯丝核菌和菌核菌对人参总皂苷化学趋向性的响应  

Chemotaxis response of Rhizoctonia solani and Sclerotinia schinsengto total ginsenosides

作　　者：张爱华[1] 匙坤 许永华[1] 孙佳明[2] 雷锋杰[1] 

机构地区：[1]吉林农业大学中药材学院,吉林省人参工程中心,吉林长春130118 [2]长春中医药大学生物工程研发中心,吉林长春130117

出　　处：《西北农林科技大学学报（自然科学版）》2016年第5期200-204,214,共6页Journal of Northwest A&F University(Natural Science Edition)

基　　金：国家自然科学基金项目(31100239,31200224);吉林省科技发展计划项目(20110926,20130206030YY,20140520159JH);公益性行业(农业)科研专项(201303111);吉林省现代农业产业技术体系建设项目(2013018)

摘　　要：【目的】探讨人参立枯丝核菌和菌核菌对人参总皂苷化学趋向性的响应。【方法】采用平板法测定人参立枯丝核菌和菌核菌在不同人参总皂苷质量浓度(200,20,2,0.2mg/L)、温度(10,15,20,25,30℃)和pH(5,6,7,8)下的化学趋向性响应,以及初生菌丝受此趋化影响下的生长状况。【结果】人参立枯丝核菌对25℃、pH=6、质量浓度为2mg/L的人参总皂苷表现出较强的正趋向性,而人参菌核菌则对15℃、pH=7、质量浓度为2mg/L的人参总皂苷表现出较强的正趋向性,趋化移动指数(CMI)分别为0.136和0.149。【结论】人参总皂苷作为趋化因子能够诱导人参立枯丝核菌和菌核菌产生化学趋向性响应。【Objective】This study investigated the chemotaxis response of Rhizoctonia solani and Sclerotinia schinsengtototal ginsenosides.【Method】Different concentrations of total ginsengsides（200,20,2,0.2mg/L）,culture temperatures（10,15,20,25,30℃）and pH values（5,6,7,8）were used to research the chemotaxis response of two pathogens by plate assay.The growth of primary hyphae affected by this chemotaxis was also analyzed.【Result】Rhizoctonia solani exhibited strong positive chemotaxi sagainst total ginsenosides with concentration of 2 mg/L when the culture temperature was 25 ℃ and the pH was 6.Sclerotinia schinsengexhibited strong positive chemotaxisagainst total ginsenosides with concentration of 2mg/L when the culture temperature was 15 ℃ and the pH was 7.The chemotactic migration indexes（CMI）of the two pathogens were 0.136 and 0.149,respectively.【Conclusion】Total ginsenosi desinduced chemotaxis response of Rhizoctonia solani and Sclerotinia schinsengas chemokines.

关 键 词：人参立枯丝核菌 人参菌核菌 人参总皂苷 化学趋向性 

分 类 号：S435.675[农业科学—农业昆虫与害虫防治]