针刺“四关”穴对阿尔茨海默病大鼠学习记忆及海马区β淀粉样蛋白42、白介素-1β和白介素-2的影响  

Effects of Acupuncture Stimulation of Bilateral“Hegu”(LI 4)and “Taichong”(LR 3)on Learning-memory Ability,Hippocampal Aβ42 Expression and Inflammatory Cytokines in Rats with Alzheimer's Disease

作　　者：姜美驰 梁静[2] 张玉杰[1] 王静蓉[1] 郝晋东[3] 王梅康[3] 许建阳[3] 

机构地区：[1]辽宁医学院,锦州121001 [2]河北医科大学,石家庄050017 [3]武警总医院,北京100039

出　　处：《针刺研究》2016年第2期113-118,共6页Acupuncture Research

基　　金：国家自然科学基金(No.30960529)

摘　　要：目的：观察针刺＂四关＂穴对阿尔茨海默病（AD）大鼠海马区白介素-1β（IL-1β）、白介素-2（IL-2）及β淀粉样蛋白（Aβ）42表达的影响,探讨针刺＂四关＂穴对AD的作用机制。方法：将SD大鼠随机分为假手术组、模型组、西药组、针刺组,每组12只。采用侧脑室注射链脲佐菌素制备AD模型。针刺组取＂四关＂穴（双＂合谷＂＂太冲＂）,留针15min,间隔5min行针1次,平补平泻,频率为120~150次/min,捻转角度为90°~180°,治疗6d休息1d为一疗程,共4个疗程;西药组进行盐酸多奈哌齐混悬液（0.45mg/kg）灌胃治疗。应用Morris水迷宫测试各组大鼠学习记忆能力的变化,采用免疫组织化学方法检测大鼠海马Aβ42的表达情况,采用双抗体夹心法检测IL-1β以及IL-2在大鼠海马中的含量。结果：与假手术组比较,模型组大鼠平均逃避潜伏期延长（P0.05）,而海马区Aβ42的阳性细胞表达和IL-1β含量降低（P〈0.05）,IL-2含量升高（P〈0.05）。结论：针刺＂四关＂穴能一定程度上改善AD大鼠学习记忆能力,其机制可能与降低海马中IL-1β含量、升高IL-2含量,以及改善Aβ沉积有关,且作用效果与西药盐酸多奈哌齐相当。Objective To observe the effect of acupuncture stimulation of bilateral＂Hegu＂（LI 4）and ＂Taichong＂（LR 3,the so-called＂Four Gate Points＂）on learning-memory ability,hippocampal interleukin-1（IL-1）βand IL-2and amyloidβ（Aβ）42levels in Alzheimer＇s disease（AD）rats,so as to reveal its underlying mechanism in improving AD.Methods Male SD rats were randomly divided into sham operation,model,medication and acupuncture groups（n=12rats in each group）.The AD model was created by microinjection of streptozotocin（10μL,3mg/kg）into the lateral ventricle（repeated the microinjection once two days later）.Bilateral LR 3and LI 4were punctured with filiform needles and stimulated manually,once a day,6days a week for 4weeks.The rats of the medication group were intragastric perfusion of Donepezil HCl（0.45mg/kg）,once a day for 4weeks.The learning-memory ability was detected by Morris water maze swimming tests.The immunoactivity of hippocampal Aβ42was detected by immunohistochemistry,and the contents of IL-1βand IL-2in the hippocampus tissue were determined by ELISA.Results After modeling,the average escape latency of Morris water navigation task was significantly increased,and the target-platform crossing times of space probe trials were significantly reduced in the model group（P0.05）,suggesting a lowering of learning-memory ability.After acupuncture intervention,the increased escape latency and the decreased target-platform crossing times were reversed,suggesting an improvement of the learning-memory.The hippocampal Aβ42immunoactivity and IL-1βcontent were significantly higher in the model group than in the sham operation group（P0.05）,but the hippocampal IL-2content was markedly decreased in the model group（P0.05）.Following the interventions,the increased Aβ42expression and IL-1βcontents,and the decreased IL-2contents in the hippocampus were also reversed in both the acupuncture and medication groups（P0.05）.Conclusion Acupuncture may improve learning-memory abili

关 键 词：阿尔茨海默病 针刺 学习记忆能力 海马 β淀粉样蛋白42 白介素-1Β 白介素-2 

分 类 号：R245.3[医药卫生—针灸推拿学]