星点-效应面法优化芍药苷醇质体制备工艺  

Preparationand formula optimization of Paeoniflorin ethosomes by Box-Behnken design and response surface method

作　　者：冯健男[1] 杜守颖[1] 白洁[1] 陆洋[1] 李鹏跃[1] 武慧超[1] 

机构地区：[1]北京中医药大学中药学院,硕士研究生100102

出　　处：《环球中医药》2016年第4期409-413,共5页Global Traditional Chinese Medicine

基　　金：国家科技重大专项重大新药创制专项(2014ZX09301306-009)

摘　　要：目的 Box-Behnken效应面法优化芍药苷醇质体的制备工艺。方法采用逆相蒸发法制备芍药苷醇质体。以磷脂与胆固醇比例(X_1)、磷脂浓度(X_2)、芍药苷浓度(X_3)为考察因素,包封率(Y)为评价指标,采用Box-Behnken效应面法对处方工艺进行优化。结果最佳处方为磷脂与胆固醇比例(mg/mg)=5.0,磷脂浓度(mg/m L)=18.0 mg/m L,芍药苷浓度(mg/m L)=5.2 mg/m L。包封率为(44.27±0.27)%,标准偏差均小于10%;粒径为(167.77±14.91)nm,多分散系数为(0.41±0.20)(n=3),变形性良好。结论采用Box-Behnken效应面法对芍药苷醇质体处方进行优化是可行和有效的。Objective To optimize the preparation technique for Paeoniflorin ethosome by Box-Behnken design and response surface method. Methods Paeoniflorin ethosomes was prepared by reverse phase evaporation. The effects of SPC- Cholesterol（ X_1）,concentration of SPC（ X_2） and concentration of Paeoniflorin（ X_3） were observed as metrics. The encapsulation efficiency（ Y） was evaluated and the formula was optimized by Box-Behnken design and response surface method. Results The optimal formula was ：X1= 5. 0,X_2= 18. 0,and X3= 5. 2. The encapsulation efficiency was（ 44. 27 ± 0. 27） %. The standardized deviations was below 10%. The average particle size was（ 167. 77 ± 14. 91） nm,PDI was（ 0. 41 ± 0. 20）（ n = 3）,deformability was satisfactory. Conclusion Using Box-Behnken design and response surface method to prepare Paeoniflorin ethosomes is feasible and effective.

关 键 词：芍药苷 醇质体 BOX-BEHNKEN效应面法 粒径 变形性 

分 类 号：R283[医药卫生—中药学]