佛山地区无偿献血人群人细小病毒B19感染情况分析  

Human parvovirus B19 detection in voluntary blood donors of Foshan City

作　　者：严军雄 伍伟健[2] 周健欣[2] 卢瑾[2] 

机构地区：[1]佛山市第四人民医院,广东佛山528000 [2]佛山市中心血站,广东佛山528000

出　　处：《国际检验医学杂志》2016年第8期1039-1040,1043,共3页International Journal of Laboratory Medicine

基　　金：广东省佛山市卫生和计划生育局科技项目(2014160)

摘　　要：目的了解佛山地区无偿献血人群人细小病毒(HPV)B19感染现状。方法采用ELISA检测血液中的HPV B19IgG和IgM抗体,并用PCR检测抗体阳性标本HPV B19DNA。结果 368例无偿献血者标本中检出HPV B19IgG阳性92例,阳性率为25.00%;检出HPV B19IgM阳性2例,阳性率为0.54%,两者比较差异有统计学意义(P<0.01)。94例抗体阳性标本中,检测HPV B19DNA阳性4例,阳性率为4.26%。结论佛山地区无偿献血人群存在较高的HPV B19既往感染率,急、慢性感染率较低,慢性感染者HPV B19病毒载量较低。Objective To investigate the infection status of human parvovirus B19（HPV B19）in voluntary blood donors of Foshan City.Methods The HPV B19 IgG and IgM antibodies of voluntary blood donors were detectd by using ELISA.Real-time PCR assay was applied to detect HPV B19 DNA on HPV B19 IgG and/or IgM antibody positive specimens.Results There were 92 HPV B19 IgG positive cases in 368 specimens,positive rate was 25.00%;and the HPV B19 IgM positive cases were 2,positive rate was 0.54%.4positive cases were detected in 94 antibody positive specimens,positive rate was 4.26%.Conclusion HPV B19 infection of blood donation person in Foshan is high,new infections and chronic infection rate are low.HPV B19 viral loads are low in people with chronic infection.

关 键 词：无偿献血者 人类细小病毒B19 血清学检测 实时荧光定量PCR 

分 类 号：R446.6[医药卫生—诊断学]