基于纳米铂-单壁碳纳米管-壳聚糖的癌胚抗原电化学阻抗型免疫传感研究  

EIS immunosensor based on PtNPs-SWCNTs-CS for detection of carcinoembryonic antigen

作　　者：金晶[1,2] 李群芳[1,2] 张升晖[1,2] 

机构地区：[1]湖北民族学院化学与环境工程学院,恩施445000 [2]湖北民族学院生物资源保护与利用湖北省重点实验室,恩施445000

出　　处：《分析试验室》2016年第4期373-377,共5页Chinese Journal of Analysis Laboratory

基　　金：湖北民族学院博士启动基金(MY2014B014);国家自然科学基金地区基金(21361009)项目资助

摘　　要：将单壁碳纳米管-壳聚糖的复合材料（SWCNTs-CS）滴涂在玻碳电极后,采用恒电位沉积的方法在SWCNTs-CS上生成纳米铂,形成Pt NPs-SWCNTsCS复合物后用以固定癌胚抗原抗体（anti-CEA）制成免疫传感界面,在癌胚抗原（CEA）的存在下,在电极表面形成抗原-抗体免疫复合物,以[Fe（CN）6]3-/4-作为混合探针电解质,构建一种简单灵敏无标记的阻抗型免疫分析方法。随着固定CEA量增加,传感器上传递电子的能力下降导致阻抗值增大,且CEA浓度在0.010~12 ng/m L范围时,与阻抗值呈现出良好的线性关系,线性方程为△Retohm=（831.3±17.8）cCEA（ng/m L）＋（885.8±95.1）（R2=0.997）,检测限为4.2 pg/m L（S/N=3）。该免疫传感器具有良好的稳定性和选择性,回收率在95.0%~108.6%,可初步用于临床对CEA的检测。A feasible and practicable electrochemical impedance immunosensor strategy was established for detection of carcinoembryonic antigen based on platinum nanoparticles assembled on functional single-walled carbon nanotubes-chitosan composites（ SWCNTs-CS） through potentiostatic deposition method,designed as Pt NPs-SWCNTs-CS composites. Pt NPs and functional SWCNTs-COOH combined with carcinoembryonic antigen antibody（ CEA） through covalent bond to form anti-CEA-Pt NPs-SWCNTs-CS / GCE. Under the existence of CEA,a simple,sensitive and untagged impedance type sensor is established with [Fe（ CN）6]3- /4-as redox medium. Under optimal conditions,with the increase of concentration of CEA,the transfer electron ability of electrodes declined,thus impedance value increased. The linear range of the resulted immunosensor was observed from 0. 01 to 12 ng / m L with a detection limit of 4. 2 pg / m L（ S / N = 3）. The immunesensor had good stability and selectivity with the recovery rate of 95. 0% to 108. 6%. It could be anticipated that this method would be used for clinical detection of CEA.

关 键 词：单壁碳纳米管 纳米铂 壳聚糖 癌胚抗原 电化学阻抗型免疫传感器 

分 类 号：O657.1[理学—分析化学]