高氧暴露对新生SD大鼠肺泡内白细胞介素-8及肿瘤坏死因子-α含量的影响  

Influence on the contents of interleukin-8 and tumor necrosis factor-αin pulmonary alveoli of neonatal rats exposed to hyperoxia

作　　者：李芳芳[1] 魏学功[1] 王霞[1] 韩素灿 张传厚[1] 张先娟[1] 李莉[1] 

机构地区：[1]滨州医学院附属医院产科,山东256603

出　　处：《中华妇幼临床医学杂志（电子版）》2016年第2期179-183,共5页Chinese Journal of Obstetrics & Gynecology and Pediatrics(Electronic Edition)

基　　金：山东省医药卫生科技发展计划项目(2014WS0487)~~

摘　　要：目的探讨新生SD大鼠高氧暴露后肺泡内白细胞介素(IL)-8及肿瘤坏死因子(TNF)-α的含量变化。方法选择100只生后24h的足月、健康SD大鼠为研究对象,按照随机数字表法,将其分为高氧组(n=50)及空气组(n=50)。高氧组大鼠持续吸入浓度≥95%的氧气,空气组大鼠置于空气中,并于给氧后第1、3、6、10、14天,每组每次各取10只大鼠进行肺泡灌洗,应用双抗体夹心酶联免疫吸附测定(ELISA)法检测肺泡灌洗液中IL-8及TNF-α含量。统计学比较两组新生SD大鼠肺泡灌洗液中IL-8及TNF-α含量差异,以及高氧组大鼠上述不同时间点IL-8及TNF-α含量差异。两组大鼠性别构成比及出生体重比较,差异无统计学意义(P>0.05)。实验过程中对动物的处置符合动物伦理学标准。结果 1高氧组大鼠在高氧暴露第14天出现生长迟缓,呆滞,体重不增,呼吸浅快,口唇发绀等不良表现。2高氧组新生SD大鼠第3、6、10、14天肺泡灌洗液中IL-8含量较空气组高[(262.4±12.5)×10^(-15) g/L vs(94.0±12.5)×10^(-15) g/L,(374.1±14.8)×10^(-15) g/L vs(94.8±7.2)×10^(-15) g/L,(345.7±17.5)×10^(-15) g/L vs(94.5±4.3)×10^(-15) g/L,(295.5±26.6)×10^(-15) g/L vs(95.6±7.5)×10^(-15) g/L],且差异有统计学意义(t=36.909、58.033、43.981、22.878,P<0.05);高氧组新生SD大鼠第1、3、6、10、14天肺泡灌洗液中TNF-α含量较空气组高[(43.0±4.5)×10^(-15) g/L vs(29.9±1.7)×10^(-15) g/L,(59.3±7.0)×10^(-15) g/L vs(33.3±4.1)×10^(-15) g/L,(55.1±7.7)×10^(-15) g/L vs(31.1±2.5)×10^(-15) g/L,(43.2±5.1)×10^(-15) g/L vs(31.2±3.4)×10^(-15) g/L,(34.4±3.8)×10^(-15) g/Lvs(30.7±2.8)×10^(-15) g/L],且差异有统计学意义(t=-6.287、-10.190、-7.358、-2.508、-4.516,P<0.05)。3高氧组新生SD大鼠肺泡灌洗液中IL-8含量于第6天均分别较第1、3、10、14天高(t=-50.812、-18.265、-3.910、-8.170,P<0.05),第10天较第14天高(t=-4.987,P<0.05),且差异均有统计学意义;高氧组新生SD大鼠肺泡灌洗液中TNObjective To explore the variation of interleukin（IL）-8 and tumor necrosis factor （TNF）-a contents in pulmonary alveoli of neonatal SD rats with hyperoxia exposed. Methods A total of 100 Sprague-Dawley（SD） rats which were healthy and 24 h after birth, were chosen as study objects. They were randomly divided into hyperxia group and air group, and 50 rats in each. Neonatal SD rats in hyperxia group were exposed to the oxygen concentration over or equal to 95%, while neonatal SD rats in air group were raised normally in air. Ten neonatal SD rats in each group were sacrificed at the lst,3rd,6th, 10th and 14th day after oxygen expose, and underwent alveolar wash. The contents of IL-8 and TNF-~ in the bronchoalveolar lavage fluid were detected by double-antibody sandwich enzyme-linked immunosorbent assay （ELISA）. The differences of IL-8 and TNF-a contents in the bronchoalveolar lavage fluid between two groups of neonatal SD rats, and the differences of IL-8 and TNF-a contents among different time points above mentioned in hyperxia group were statistically compared. There were no significant differences between two groups of neonatal SD rats in gender constituent ratio and birth weight （P〈0.05）. The disposition of the animals in experimental process was in accordance with the ethical standard of animals. Results （i） Neonatal SD rats in hyperxia group had some bad performances on 14th day of hyperoxia expose, such as growth retardation, sluggishness, weight gain stopped, rapid and shallow breathing and cyanosis of oral lips,etc.. The contents of IL-8 in the bronchoalveolar Iavage fluid of neonatal SD rats on 3rd,6th, 10th, 14th days in hyperxia group were higher than those of air group[（262.4±12.5） × 10 is g/L vs （94.0±12.5）× 10 15 g/L,（374.1±14.8）×10-1 g/L vs （94.8±7.2）×10-15 g/L,（345. 7±17.5）×10 ls g/L vs （94.5±4.3）× 10 15^ g/L, （295.5±26.6） X 10 lS g/L vs （95.6±7.5） × 10 15 g/L],and the differences were statistically significant（t = 36. 909,58.

关 键 词：高氧症 肺损伤 支气管肺泡灌洗液 白细胞介素8 肿瘤坏死因子α大鼠 SPRAGUE-DAWLEY 

分 类 号：R722.1[医药卫生—儿科]