徐长卿丹皮酚对兔膝关节软骨细胞凋亡及相关基因的影响  

Effects of Cynanchum Paeonol on Apoptosis and Related Genes in Rabbit Knee Cartilage

作　　者：吴琪[1] 任婕[1] 

机构地区：[1]湖北中医药大学,湖北武汉430061

出　　处：《湖北中医药大学学报》2016年第2期11-14,共4页Journal of Hubei University of Chinese Medicine

摘　　要：目的观察徐长卿丹皮酚对兔膝关节软骨细胞凋亡及其调控基因P53、Bcl-2表达作用的影响。方法将40只大耳白兔随机分为正常组、模型组、生理盐水组、曲安奈德组、徐长卿丹皮酚组。采用内侧半月板前1/3切除制作骨关节炎模型,造模成功后,给药组关节腔注射相应药物,给药5周后取材。两步法免疫组化染色观察关节软骨形态变化,原位末端标记检测关节软骨细胞凋亡率,免疫荧光检测软骨组织P53、Bcl-2蛋白表达。结果模型组Mankin评分、软骨细胞凋亡率明显升高(P<0.01)。而徐长卿丹皮酚组和曲安奈德组软骨组织Mankin评分、软骨细胞凋亡率相比模型组降低(P<0.05),但仍高于正常组(P<0.01)。模型组软骨组织中P53、Bcl-2表达率均升高(P<0.05,P<0.01)。徐长卿丹皮酚组和曲安奈德组软骨组织中Bcl-2表达升高,而P53表达率降低(P<0.05),其中徐长卿丹皮酚组升高Bcl-2的效果更显著(P<0.01)。结论徐长卿丹皮酚能在一定程度上抑制骨性关节炎软骨细胞的过度凋亡,其对损伤关节软骨的修复作用可能与调节P53和Bcl-2的表达有关。Objective To study the effcts of Cynanchum Paeonol on apoptosis and expression of regulatory gene P53 and Bcl- 2 in rabbit knee cartilage cell. Methods 40 rabbits were randomly divided into normal group,control group,saline group,Triamcinolone group,Cynanchum Paeonol group. The model was produced by the method of ACLT and medial meniscus resection 1 /3 osteoarthritis. After the models established successfully,each administration group was given articular injection with appropriate medication,the administration was harvested after 5 weeks. Use 2- step immunohistochemical staining to observe articular cartilage morphology,use TUNEL to detect apoptosis rate of articular cartilage,use cartilage tissue immunofluorescence to detect P53 and Bcl- 2 protein expression. Results Mankin score,chondrocyte apoptosis rate of model group significantly increased（ P〈0. 01）. Cartilage Mankin score,chondrocyte apoptosis rate of Cynanchum Paeonol group and Triamcinolone group compared to model group decreased（ P〈0. 05）,but still higher than normal group（ P〈0. 01）. Cartilage P53,Bcl- 2 expression rate of model group increased（ P〈0. 01,P〈0. 05）. Expression rate of Bcl- 2 and P53 in Cynanchum Paeonol group and Triamcinolone group decreased（ P〈0. 05）,BCl- 2 of Cynanchum Paeonol group increased more significantly（ P〈0. 01）. Conclusion Cynanchum Paeonol can inhibit excessive apoptosis of osteoarthritis chondrocytes to a certain extent,the repairing effect of articular cartilage damage may be related to the regulation of P53 and Bcl- 2 expression.

关 键 词：徐长卿丹皮酚 骨性关节炎 凋亡 P53 BCL-2 

分 类 号：R285.5[医药卫生—中药学]